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벤질리덴아세톤 화학구조 변이에 따른 생리활성 변화 분석 및 식물 병해충 방제 효과

Structure-activity Analysis of Benzylideneacetone for Effective Control of Plant Pests

  • 서삼열 (안동대학교 자연과학대학 생명자원과학과) ;
  • 전미현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 천원수 (안동대학교 자연과학대학 생명자원과학과) ;
  • 이성홍 (안동대학교 자연과학대학 응용화학과) ;
  • 서지애 ((주)B&L agro) ;
  • 이영근 (안동대학교 자연과학대학 생명자원과학과) ;
  • 홍용표 (안동대학교 자연과학대학 응용화학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University) ;
  • Jun, Mi-Hyun (Department of Bioresource Sciences, Andong National University) ;
  • Chun, Won-Su (Department of Bioresource Sciences, Andong National University) ;
  • Lee, Sung-Hong (Department of Applied Chemistry, Andong National University) ;
  • Seo, Ji-Ae (B&L agro) ;
  • Yi, Young-Keun (Department of Bioresource Sciences, Andong National University) ;
  • Hong, Yong-Pyo (Department of Applied Chemistry, Andong National University) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 투고 : 2011.03.22
  • 심사 : 2011.04.26
  • 발행 : 2011.06.30

초록

벤질리덴아세톤은 곤충병원세균인 Xenorhabdus nematophila의 배양액에서 유래된 물질이다. 벤질리덴아세톤은 아이코사노이드 생합성을 억제하여 곤충의 면역을 저하시키는 것으로 알려져 있으며, 이 물질을 미생물농약에 첨가하면 병원성의 제고 효과를 기대할 수 있다. 본 연구는 벤질리덴아세톤의 면역억제 능력을 제고시킬 목적으로 이 물질의 소수성을 낮추는 유도체를 화학 합성하였다. 수산기를 첨가한 두 가지 벤질리덴아세톤 유도체와 설탕이 부착된 벤질리덴아세톤 유도체가 각각 합성되었다. 이 유도체들은 모두 배추좀나방(Plutella xylostella)의 phospholipase $A_2$ ($PLA_2$)와 phenoloxidase (PO) 활성을 모두 억제하였으며, 이 가운데 벤질리덴아세톤이 가장 억제력이 높았다. 이러한 벤질리덴아세톤 유도체들을 각각 Bacillus thuringiensis (Bt) 생물농약과 혼합하면 미생물의 병원성을 증가시켰다. 벤질리덴아세톤은 또한 네 가지 식물병원성 진균의 성장을 억제시켰다. 그러나 이 물질의 유도체들(특히 설탕 중합체)의 병원균 성장 억제 능력은 일부 감소했다. 이러한 결과는 벤질리덴아세톤과 벤질리덴아세톤 유도체는 면역작용에 관여하는 $PLA_2$와 PO의 두 가지 효소 활성을 억제하며, 배추좀나방에 대한 Bt 병원성을 제고시켰으며, 식물병원성 진균에 대한 항균제로서의 개발 가능성을 제시하고 있다.

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