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Structure-activity Analysis of Benzylideneacetone for Effective Control of Plant Pests

벤질리덴아세톤 화학구조 변이에 따른 생리활성 변화 분석 및 식물 병해충 방제 효과

  • Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University) ;
  • Jun, Mi-Hyun (Department of Bioresource Sciences, Andong National University) ;
  • Chun, Won-Su (Department of Bioresource Sciences, Andong National University) ;
  • Lee, Sung-Hong (Department of Applied Chemistry, Andong National University) ;
  • Seo, Ji-Ae (B&L agro) ;
  • Yi, Young-Keun (Department of Bioresource Sciences, Andong National University) ;
  • Hong, Yong-Pyo (Department of Applied Chemistry, Andong National University) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 서삼열 (안동대학교 자연과학대학 생명자원과학과) ;
  • 전미현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 천원수 (안동대학교 자연과학대학 생명자원과학과) ;
  • 이성홍 (안동대학교 자연과학대학 응용화학과) ;
  • 서지애 ((주)B&L agro) ;
  • 이영근 (안동대학교 자연과학대학 생명자원과학과) ;
  • 홍용표 (안동대학교 자연과학대학 응용화학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2011.03.22
  • Accepted : 2011.04.26
  • Published : 2011.06.30

Abstract

Benzylideneacetone (BZA) is a compound derived from culture broth of an entomopathogenic bacterium, Xenorhabdus nematophila (Xn). Its immunosuppressive activity is caused by its inhibitory activity against eicosanoid biosynthesis. This BZA is being developed as an additive to enhance control efficacy of other commercial microbial insecticides. This study was focused on the enhancement of the immunosuppressive activity of BZA by generating its chemical derivatives toward decrease of its hydrophobicity. Two hydroxylated BZA and one sugar-conjugated BZA were chemically synthesized. All derivatives had the inhibitory activities of BZA against phospholipase $A_2$ ($PLA_2$) and phenoloxidase (PO) of the diamondback moth, Plutella xylostella, but BZA was the most potent. Mixtures of any BZA derivative with Bacillus thuringiensis (Bt) significantly increased pathogenicity of Bt. BZA also inhibited colony growth of four plant pathogenic fungi. However, BZA derivatives (especially the sugar-conjugated BZA) lost the antifungal activity. These results indicated that BZA and its derivatives inhibited catalytic activities of two immune-associated enzymes ($PLA_2$ and PO) of P. xylostella and enhanced Bt pathogenicity. We suggest its use to control plant pathogenic fungi.

Keywords

Benzylideneacetone;Plutella xylostella;Plant pathogen;Phospholipase $A_2$;Phenoloxidase

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