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Analysis of the Expression Patterns of Thymosin β4, Vascular Endothelial Growth Factor, and Hypoxia-Inducible Factor-1α in Various Tumors Using Tissue Microarray

Tissue microarray를 이용한 여러 암에서의 thymosin β4, vascular endothelial growth factor, 및 hypoxia-inducible factor-1α 발현양상 연구

  • Lee, Bo-Young (Department of Parasitology and Gnenetics, Kosin University College of Medicine) ;
  • Lee, Seung-Hyun (Department of Surgery, Kosin University College of Medicine) ;
  • Ahn, Byung-Kwon (Department of Surgery, Kosin University College of Medicine) ;
  • Ock, Mee-Sun (Department of Parasitology and Gnenetics, Kosin University College of Medicine) ;
  • Cha, Hee-Jae (Department of Parasitology and Gnenetics, Kosin University College of Medicine)
  • 이보영 (고신대학교 의과대학 기생충학 유전학 교실) ;
  • 이승현 (고신대학교 의과대학 외과학 교실) ;
  • 안병권 (고신대학교 의과대학 외과학 교실) ;
  • 옥미선 (고신대학교 의과대학 기생충학 유전학 교실) ;
  • 차희재 (고신대학교 의과대학 기생충학 유전학 교실)
  • Received : 2010.12.23
  • Accepted : 2010.12.30
  • Published : 2011.03.30

Abstract

Thymosin ${\beta}4$ (TB-4) has been reported to play a key role in tumor growth, metastasis and angiogenesis. In addition, TB-4 induced the expression of vascular endothelial growth factor (VEGF) and stabilized the hypoxia-inducible factor (HIF)-$1{\alpha}$ in melanoma cells. Although the importance of thymosin ${\beta}4$ in angiogenesis and metastasis has been proven, there are few studies that show the expression patterns of TB-4, VEGF and HIF-$1{\alpha}$. This study was conducted to analyze the relationship among these proteins in various tumors. Using tissue microarray analysis, we investigated the expression patterns of TB-4, VEGF and HIF-$1{\alpha}$ in various tumors to identify the expression patterns and relationships of these proteins in certain tumors. TB-4 was highly expressed in osteosarcoma, colon adenocarcinoma, esophageal squamous cell carcinoma, kidney and urinary bladder transitional carcinoma, lung cancer, and liver cancer. HIF-$1{\alpha}$ was highly expressed in nasal cavity inverted papilloma, lung cancer, and esophageal squamous cell carcinoma. The expression patterns of TB-4 and HIF-$1{\alpha}$ were almost similar and co-localized. VEGF expression was high in the blood vessels in tumors, but usually not high in the tumors themselves. VEGF was moderately expressed in stomach cancer, liver angiosarcoma, gall bladder adenocarcinoma, and uterus endometrial adenocarcinoma. The expression patterns of VEGF shows similarities in certain tumors including stomach cancer, osteosarcoma, liposarcoma, lung cancer, liver cancer, gall bladder adenocarcinoma, esophageal squamous cell carcinoma, stomach cancer, colorectal carcinoma and renal cell carcinoma. These results suggest that the expression patterns of TB-4, HIF-$1{\alpha}$ and VEGF were co-localized and related to tumorigenesis and angiogenesis of certain tumors.

Acknowledgement

Supported by : 한국연구재단

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