Protective Effect of HemoHIM on Epidermal Melanocytes in Ultraviolet-B irradiated Mice

자외선 B 조사 마우스에서 표피멜라닌세포 변화에 대한 헤모힘의 방어효과

  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Jung, U-Hee (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Jang, Jong-Sik (Department of Animal Science, Kyungpook National University) ;
  • Kim, Tae-Hwan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Received : 2011.05.19
  • Accepted : 2011.06.24
  • Published : 2011.06.30


We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet-B (UV-B) irradiation, and observed the effect of an herbal preparation (HemoHIM, HH) on the formation, and decrease of UV-B-induced epidermal melanocytes. C57BL/6 mice were irradiated by UV-B $80\;mJ{\cdot}cm^{-2}$ ($0.5\;mW{\cdot}sec^{-1}$) daily for 7 days, and HH was intraperitoneally, orally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 13~15 melanocytes${\cdot}mm^{-2}$, and one week after UV irradiation, the applied areas showed an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal, oral or topical treatment with HH before each irradiation interrupted UV-B-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to the number found in UV-B-irradiated, untreated control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with HH at 3rd and 6th weeks after irradiation. The present study suggests the HH as inhibitor of UV-B-induced pigmentation, and depigmenting agent.


Supported by : 한국과학재단, 전남대학교 동물의학연구소


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