Protective Effect of HemoHIM on Epidermal Melanocytes in Ultraviolet-B irradiated Mice

자외선 B 조사 마우스에서 표피멜라닌세포 변화에 대한 헤모힘의 방어효과

  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Jung, U-Hee (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Jang, Jong-Sik (Department of Animal Science, Kyungpook National University) ;
  • Kim, Tae-Hwan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Received : 2011.05.19
  • Accepted : 2011.06.24
  • Published : 2011.06.30

Abstract

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet-B (UV-B) irradiation, and observed the effect of an herbal preparation (HemoHIM, HH) on the formation, and decrease of UV-B-induced epidermal melanocytes. C57BL/6 mice were irradiated by UV-B $80\;mJ{\cdot}cm^{-2}$ ($0.5\;mW{\cdot}sec^{-1}$) daily for 7 days, and HH was intraperitoneally, orally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 13~15 melanocytes${\cdot}mm^{-2}$, and one week after UV irradiation, the applied areas showed an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal, oral or topical treatment with HH before each irradiation interrupted UV-B-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to the number found in UV-B-irradiated, untreated control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with HH at 3rd and 6th weeks after irradiation. The present study suggests the HH as inhibitor of UV-B-induced pigmentation, and depigmenting agent.

Acknowledgement

Supported by : 한국과학재단, 전남대학교 동물의학연구소

References

  1. Kaufman RJ. Vectors used for expression in mammalian cells. Meth. In. Enzymol. 1991;205: 87-92.
  2. Weixiong L, Helene ZH. Induced melanin reduces mutations and killing in mouse melanoma. Phytochem. Phytobiol. 1997;65:480-484. https://doi.org/10.1111/j.1751-1097.1997.tb08594.x
  3. Maeda K. Fukada M. In vivo effectiveness of several whitening cosmetic components in human melanocytes. J. Soc. Cosmet. Chem. 1991;42: 361-368.
  4. Dawley RM, Flurkey WH. 4-hexylresocinol, a potent inhibitor of mushroom tyrosinase. J. Food Sci. 1993;58:609-610. https://doi.org/10.1111/j.1365-2621.1993.tb04336.x
  5. Tomita K, Oda N, Ohbayashi M, Kamei H, Miyaki T, Oki T. A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis. J. Antibiot. (Tokyo) 1990;43:1601-1605. https://doi.org/10.7164/antibiotics.43.1601
  6. Laskin JD, Piccinini LA. Tyrosinase isozyme heterogeneity in differentiating B16/C3 melanoma. J. Biol. Chem. 1986;261:16626-16635.
  7. Naeyaert JM, Eller M, Gordon PR, Park HY, Gilchrest BA. Pigment content of cultured human melanocytes does not correlate with tyrosinase message level. Br. J. Dermatol. 1991;125:297-303. https://doi.org/10.1111/j.1365-2133.1991.tb14161.x
  8. Jo SK, Park HR, Jung UH, Oh H, Kim SH, Yee ST. Protective effect of a herbal preparation (HemoHIM) on the selfrenewal tissues and immune system against ɤ-irradiation. J. Korean Soc. Food Sci. Nutr. 2005;34:805813.
  9. Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M. Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res. 1998;11: 206-212. https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  10. Kahn V. Effect of kojic acid on the oxidation of DL-DOPA, norepinephrine, and dopamine by mushroom tyrosinase. Pigment Cell Res. 1995;8: 234-240. https://doi.org/10.1111/j.1600-0749.1995.tb00669.x
  11. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J. Agric. Food Chem. 2003;51:1201-1207. https://doi.org/10.1021/jf020935u
  12. Shin NH, Ryu SY, Choi EJ, Kang SH, Chang IM, Min KR, Kim Y. Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase. Biochem. Biophys. Res. Commun. 1998;243:801-803. https://doi.org/10.1006/bbrc.1998.8169
  13. Kim JA, Choi JY, SoN AR, Park SH, Xu GH, Lee JG, Oh IS, Kim JJ, Chang HW, Chung SR, Jang TS, Lee SH. Inhibitory effect of some natural polyphenols isolated from euphorbiaceae plants on melanogenesis. Kor. J. Pharmacogn. 2004;35:157- 163.
  14. Kim CT, Kim WC, Jin MH, Kim HJ, Kang SJ, Kang SH, Jung MH, Lim YH. Inhibitors of melanogenesis from the roots of Peucedanum praeruprorum. Kor. J. Pharmacogn. 2002;33:395-398.
  15. Choi DY, Ahn SY, Lee SG, Han JS, Kim EC, Lee HB, Shin JH, Kim EK, Row KH. Separation and performance test of whitening agent in Rhodiola sachalinensis. Korean J. Biotechnol. Bioeng. 2004; 19:169-173.
  16. Chun HJ, Hwang SG, Lee JS, Baek SH, Jeon BH, Woo WH. Inhibitory effects of butyl alcohol extract from Caesalpinia sappan L. on melanogenesis in melan-a cells. Kor. J. Pharmacogn. 2002;33: 130-136.
  17. Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 2004;84:1155- 1228. https://doi.org/10.1152/physrev.00044.2003
  18. Gerson DE, Szabo G. Effect of single gene substitution on the melanocyte system of the C57BL mouse: quantitative and qualitative histology. Nature. 1968;218:381-382. https://doi.org/10.1038/218381a0
  19. Reynolds J, The epidermal melanocytes of mice. J. Anat. 1954;88:45-58.
  20. Jimbow K, Uesugi T. New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells. J. Invest. Dermatol. 1982;78:108-115. https://doi.org/10.1111/1523-1747.ep12505758
  21. Black HS. Reassessment of a free radical theory of cancer with emphasis on ultraviolet carcinogenesis. Integr. Cancer Ther. 2004;3:279-293. https://doi.org/10.1177/1534735404270612
  22. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T. UV-induced skin damage. Toxicology. 2003;189:21-39. https://doi.org/10.1016/S0300-483X(03)00150-1
  23. Pelle E, Huang X, Mammone T, Marenus K, Maes D, Frenkel K. Ultraviolet-B-induced oxidative DNA base damage in primary normal human epidermal keratinocytes and inhibition by a hydroxyl radical scavenger. J. Invest. Dermatol. 2003;121:177-183. https://doi.org/10.1046/j.1523-1747.2003.12330.x
  24. Zastrow L, Groth N, Klein F, Kockott D, Lademann J, Ferrero L. UV, visible and infrared light. Which wavelengths produce oxidative stress in human skin? Hautarzt 2009;60:310-317. https://doi.org/10.1007/s00105-008-1628-6
  25. Jagetia GC. Radioprotective potential of plants and herbs against the effects of ionizing radiation. J. Clin. Biochem. Nutr. 2007;40:7481.
  26. Kim SH, Kim JC, Kim SR, Lee HJ. Evaluation on biosafety in long-term administration, teratogenicity and local toxicity of developed product. KAERI/CM-867-2004.