어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율

  • Kim, Seon-Mi (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Choi, Nam-Ki (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Cho, Seong-Hoon (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Lee, Seok-Woo (Department of Periodontics and Dental education, School of Dentistry, Chonnam National University) ;
  • Lim, Hoi-Jeong (Department of Orthodontics, School of Dentistry, Chonnam National University) ;
  • Lim, Hoi-Soon (Department of Dental education, School of Dentistry, Chonnam National University) ;
  • Kang, Mi-Sun (Department of Microbiology, College of Medicine, Chonnam National University) ;
  • Oh, Jong-Suk (Department of Microbiology, College of Medicine, Chonnam National University)
  • 김선미 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 최남기 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 조성훈 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 이석우 (전남대학교 치의학전문대학원 치의학교육학교실 및 치주학교실) ;
  • 임회정 (전남대학교 치의학전문대학원 교정학교실) ;
  • 임회순 (전남대학교 치의학전문대학원 치의학교육학교실) ;
  • 강미선 (전남대학교 의과대학 미생물학교실) ;
  • 오종석 (전남대학교 의과대학 미생물학교실)
  • Received : 2011.04.13
  • Accepted : 2011.05.10
  • Published : 2011.05.31


The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, $bla_{SHV}$, $bla_{TEM}$, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, $bla_{TEM}$ and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. $bla_{SHV}$ associated with resistance to ${\beta}$-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and $bla_{SHV}$. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).


Periodontopathic bacteria;Antibiotic resistance;Antibiotic resistance determinants;Oral biofilm;Polymerase chain reaction


Supported by : 전남대학교


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