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Genetic polymorphism in regulatory region of fatty acid binding protein 4 (FABP4) and its effect on carcass weight in Hanwoo steers

한우 지방산결합단백질 4(FABP4) 유전자 조절영역내 단일염기변이(SNP)와 도체형질간 연관성 분석

  • Lee, Seung-Hwan (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Nam-Kuk (National Agricultural Product Quality Management Service) ;
  • Kim, Seung-Chang (Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Choi, Bong-Hwan (Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Heo, Kang-Neung (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Lee, Chang-Soo (Kon-Kuk University) ;
  • Kim, Oun-Hyun (Kon-Kuk University) ;
  • Lee, Jun-Heon (Department of Animal Science, Chung Nam Nat'l University) ;
  • Kim, Hyeong-Cheul (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Hong, Seong-Koo (Hanwoo Experiment Station, National Institute of Animal Science, RDA)
  • Received : 2011.10.13
  • Accepted : 2011.12.18
  • Published : 2011.12.31

Abstract

The aim of this study was to identify the polymorphism on fatty acid binding protein (FABP4) gene promoter region and its association with carcass traits in Hanwoo. We performed PCR-direct sequencing of FABP4 promoter region to identify single nucleotide polymorphism (SNPs) using unrelated 24 Hanwoo bulls. Four SNPs (-298A>G, -472A>G, -887A>G, -862A>G) were detected in the promoter region and genotyped on 583 Hanwoo steers. A linear mixed model revealed an association of three SNPs (-298A>G, -472A>G and -862A>G) with carcass weight and marbling score in dominance model (P<0.05). The animals with AA genotypes for the three SNPs were heavier carcass weight (5 kg) than animals with GG genotypes in the statistical analysis. For the marbling score, the AA genotype was lower effect of marbling score (0.21) than GG genotypes. In conclusion, this study indicates an important role for three SNPs detected in promoter region of FABP4 in determining carcass weight and marbling score in Hanwoo.

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