Analysis of RNA Polymerase Beta Subunit (rpoB) Gene Sequences for the Discrimination of Cyanobacteria Anabaena Species

남조세균 Anabaena 종 구분을 위한 RNA Polymerase Beta Subunit (rpoB) 유전자 염기서열 분석

  • Cheon, Ju-Yong (Department of Green Life Science, Sangmyung University) ;
  • Lee, Min-Ah (Department of Green Life Science, Sangmyung University) ;
  • Ki, Jang-Seu (Department of Green Life Science, Sangmyung University)
  • 천주용 (상명대학교 그린생명과학과) ;
  • 이민아 (상명대학교 그린생명과학과) ;
  • 기장서 (상명대학교 그린생명과학과)
  • Received : 2011.08.29
  • Accepted : 2011.09.28
  • Published : 2011.09.30


Anabaena (Cyanobacteria, Nostocales) are important for water quality controls, because they are often responsible for freshwater green tides; moreover, some species are reported to produce hepatotoxin. In this study, we sequenced RNA polymerase beta subunit (rpoB) gene of Anabaena, and evaluated their sequences for the potential use of a molecular taxonomic marker in this taxon. Anabaena rpoB showed low DNA similarity and high genetic divergences when compared those of 16S rRNA, and the molecular differences were statistically significant (Student t-test, p<0.01). Parsimony analyses showed the rpoB gene evolves 4.8-fold faster than 16S rRNA. In addition, phylogeny of the rpoB gene separated each Anabaena strain more clearly compared with a 16S rRNA tree. These results suggest that the rpoB gene is a useful marker for the molecular phylogenetics and the species discrimination of Anabaena.


Anabaena;cyanobacteria;DNA similarity;genetic distance;rpoB gene


Supported by : 상명대학교


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