Isolation, Identification, and Characterization of Ornithine-Producing Enterococcus faecalis OA18 from Kefir Grain

케피어그레인으로 제조한 요쿠르트로부터 Enterococcus faecalis OA18 균주의 분리 및 특성규명

  • Yu, Jin-Ju (Department of Biotechnology, Woosuk University Graduate School) ;
  • Kim, Su-Gon (Department of Biotechnology, Woosuk University Graduate School) ;
  • Seo, Kyoung-Won (Department of Biotechnology, Woosuk University Graduate School) ;
  • Oh, Suk-Heung (Department of Biotechnology, Woosuk University Graduate School)
  • 유진주 (우석대학교 대학원 생명공학과) ;
  • 김수곤 (우석대학교 대학원 생명공학과) ;
  • 서경원 (우석대학교 대학원 생명공학과) ;
  • 오석흥 (우석대학교 대학원 생명공학과)
  • Received : 2011.07.26
  • Accepted : 2011.09.14
  • Published : 2011.09.30


Lactic acid bacteria (LAB) OA18 was isolated from yogurt prepared by using Kefir Grain as a starter. The OA18 strain was a Gram-positive, cocci-type bacterium, and able to grow anaerobically with $CO_2$ production. The OA18 strain grew well on MRS broth supplemented with 50 mM arginine at $30-37^{\circ}C$ and pH of 7.0-9.0. The optimum temperature and pH for growth are $37^{\circ}C$ and pH 7.0. The isolate fermented ribose, D-glucose, cellobiose, D-trehalose, but not L-xylose, D-melibiose, and inositol. The 16S rRNA gene sequence of the isolate showed 99.8% homology with the Enterococcus faecalis 16S rRNA gene (Access no. AB012212). Based on the biochemical characteristics and 16S rRNA gene sequence analysis data, it was identified and named as E. faecalis OA18. The E. faecalis OA18 strain showed a high ornithine-producing capacity in the presence of arginine and also showed an antimicrobial activity against Streptomyces strains such as Streptomyces coelicolor subsp. Flavus, S. coeruleorubidus, S. coeruleoaurantiacus, S. coelicolor, S. coeruleoprunus. The cell growth of E. faecalis OA18 strain was maintained in MRS broth with a NaCl concentration of 0-7%.


E. faecalis;anti-microbial activity;Kefir grain;ornithine


Supported by : 우석대학교


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