Characterization of an Alkaline Protease from an Alkalophilic Bacillus pseudofirmus HS-54

호알칼리성 Bacillus pseudofirmus HS-54가 생산하는 알칼리성 Protease의 특성

  • Bang, Seong-Ho (Department of Biological Sciences, Hanseo University) ;
  • Jeong, In-Sil (Department of Biological Sciences, Hanseo University)
  • 방성호 (한서대학교 생명과학과) ;
  • 정인실 (한서대학교 생명과학과)
  • Received : 2011.08.19
  • Accepted : 2011.09.05
  • Published : 2011.09.30


An alkalophilic bacterium producing alkaline protease was isolated from waste water and solar saltern sample and identified as Bacillus pseudofirmus HS-54 based on morphological, biochemical characteristics as well as 16S-rRNA gene sequencing. The HS-54 protease was purified to homogeneity using ammonium sulfate precipitation, DEAE cellulose column chromatography, and sephadex G-100 gel filtration with a 4.0 purification fold. The molecular mass of the purified enzyme was estimated by SDS-PAGE to be 27 kDa. The optimal pH and temperature for the purified protease activity were 10.0 and $50^{\circ}C$, respectively. The purified enzyme was relatively stable at the pH range of 6.0-11.0 and at the temperature below $50^{\circ}C$. This enzyme was activated by $Ca^{2+}$ and $Mg^{2+}$ and inhibited by $Hg^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $Al^{3+}$, $Ag^{2+}$. And this enzyme was strongly inhibited by PMSF, suggesting that it belongs to the serine protease superfamily.


Bacillus pseudofirmus HS-54;alkaline protease;purification;serine protease


Supported by : 한서대학교


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