Identification of the SNP (Single Necleotide Polymorphism) of the Stearoyl-CoA Desaturase (SCD) Associated with Unsaturated Fatty Acid in Hanwoo (Korean Cattle)

  • Received : 2010.11.12
  • Accepted : 2011.01.19
  • Published : 2011.06.01


Fatty acid composition of beef intramuscular tissue is an important trait because high proportions of mono-unsaturated fatty acid are related to favorable beef flavor. In this study, we investigated the effects of genetic factors, such as stearoyl-CoA desaturase (SCD), on beef carcass traits, including fatty acid composition, in the Hanwoo. Analysis of fatty acids in Hanwoo was performed using a breed raised in Gyeonbuk province (n = 395). Compared to the homozygote, the GA, CT, and CT genotypes of exon 5 in the SCD polymorphism showed a higher content of oleic acid (p<0.05) and higher contents of mono-unsaturated fatty acid (p<0.05) and marbling scores (p<0.05) in intramuscular fat. Results of haplotype analysis showed a significant presence of unsaturated fatty acids and marbling score in the $ht1^*ht2$ and $ht2^*ht2$ groups (p<0.05). Furthermore, haplotype effects more powerful than a single gene were also observed. These ht1 and ht2 types also showed a significant difference in unsaturated fatty acids and marbling score, affecting beef flavor in the Hanwoo groups. Therefore, it can be inferred that the ht1 and ht2 types might be valuable new markers for use in improvement of Hanwoo.


  1. Alexander, L. J., M. D. MacNeil, T. W. Geary, W. M. Snelling, D. C. Rule and J. A. Scanga. 2007. Quantitative trait loci with additive effects on palatability and fatty acid composition of meat in a Wagyu-Limousin F2 population. Anim. Genet. 38: 506-513.
  2. Barret, J. C., B. Fry, J. Maller and M. J. Daly. 2005. Haploview: anaylsis and visualization of LD and haplotype maps. Bioinformatics 21(2):263-265.
  3. Barton, L., T. Kott, D. Bures, D. Rehak, R. Zahradkova and B. Kottova. 2009. The polymorphism of stearoyl-CoA desaturase (SCD1) and sterol regulatory element binding proyein-1 (SREBP-1) genes and their association with the fatty acid profile of muscle and subcutaneous fat in Fleckvieh bulls. Meat Sci. 9:309-330.
  4. Choi, C. B., H. U. Shin, S. O. Lee, S. I. Kim, K. K. Jung, C. W. Choi, K. H. Beak, D. K. Lunt and S. B. Smith. 2008. Comparison of cholesterol contents and fatty acid composition in M. longissimus of Hanwoo, Angus and Wagyu Crossbred Steers. J. Anim. Sci. Technol. (Kor). 50(4):519-526.
  5. Cho, S. H., J. H. Kim, J. H. Kim, P. N. Seong, B. Y. Park, K. E. Kim, Y. S. Ko, J. M. Lee and D. H. Kim. 2008. Effect of Socio-demographic Factors on Sensory Properties for Hanwoo Steer Beef with $1^{++}$ Quality Grade by Different Cut and Cooking Methods. Kor. J. Food Sci. Ani. Resour. 28(3):363-372.
  6. Chung, K. Y., D. K. Lunt, H. Kawachi, H. Yano and S. B. Smith. 2007. Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short- and long-fed Angus and Wagyu steers fed corn- or hay-based diets. J. Anim. Sci. 85:380-387.
  7. Dryden, F. D. and J. A. Marchello. 1970. Influence of total lipid and fatty acid composition upon the palatability of three bovine muscles. J. Anim. Sci. 31:36-41.
  8. Eberlr, M. A., M. J. Rieder, L. Kruglyak and D. A. Nickerson. 2006. Allele frequency matching between SNPs reveals an excess of linkage disequilibrium in genic regions of the human genome. PLoS Genetics. 9(2):1319-1327.
  9. Erkkila, A., V. D. F. de Mello, U. Riserius and D. E. Laaksonen. 2008. Dietary fatty acid and cardiovascular disease: An epidemiological approach. Prog. Lipid Res. 47(3):172-187.
  10. Folch, J., M. Lee and G. H. Sloane Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226(4):97-509.
  11. Gabriel, S. B., S. F. Schaffner, H. Nguyen, J. M. Moore, J. Roy, B. Blumenstiel, J. Higgins, M. DeFelice, A. Lochner, M. Faggart, S. N. Liu-Cordero. C. Rotimi, A. Adeyemo, R. Cooper, R. Waed, E. S. Lander, M. J. Daly and D. Altshuler. 2002. The structure of haplotype blocks in the human genome. Sciences 296:2225-2229.
  12. Hoashi, S., N. Ashida, H. Ohsaki, T. Utsugi, S. Sasazaki, M. Taniguchi, K. Oyama, F. Mukai and H. Mannen. 2007. Genotype of bovine sterol regulatory element binding protein- 1(SREBP-1) is associated with fatty acid composition in Japanese Black cattle. Mamm. Genome 18:880-886.
  13. Hoashi, S., T. Hinenoya, A. Tanaka, H. Ohsaki, H. Sasazaki, M. Taniguchi, K. Oyama, M. Mukai and H. Mannen. 2008. Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle. BMC Genetics 9:84.
  14. Ibi, T., H. Hirooka, A. K. Kahi, Y. Sasae and Y. Sasaki. 2005. Genotype environment interaction effects on carcass traits in Japanese Black cattle. J. Anim. Sci. 83:1503-1510.
  15. Jeremiah, I. E. 1996. The influence of subcutaneous fat thickness and marbling on beef. Food Res. Int. 29(5/6):513-520.
  16. Kgwatalala, P. M., E. M. Ibeagha-Awemu, J. F. Hayes and X. Zhao. 2007. Single nucleotide polymorphism in the open reading frame of the stearoyl-CoA desaturase gene and resulting genetic variants in Canadian Holstein and Jersey cows. DNA Seq. 18(5):357-362.
  17. Kuchel, H., B. D. Siebert, C. D. K. Bottema, G. C. Webb, A. M. Crawford, S. J. Duncan, P. A. McDonald, J. C. McEwan and W. S. Pitchford. 2004. Physical mapping of the stearoyl-CoA desaturase (SCD) locus in sheep. Anim. Genet. 35:163.
  18. Lee, H. I., M. H. Lee and M. S. Chung. 1994. Comparison of flavor characteristics and palatability of beef obtained from various breeds. Korean J. Food Sci. 26(5):500-506.
  19. Lee, M. A., O. M. Keane, B. C. Glass, T. R. Manley, N. G. Cullen, K. G. Dodds, A. F. McCulloch, C. A. Morris, M. Schreiber, J. Warren, A. Zadissa, T. Wilson and J. C. McEwan. 2006. Establishment of a pipeline to analyse non-synonymous SNPs in Bos Taurus. BMC Genomics 7:298.
  20. May, S. G., C. A. Sturdivant, D. K. Lunt, R. K. Miller and S. B. Smith. 1993. Comparison of sensory characteristics and fatty acid composition between Wagyu crossbred and Angus steers. Meat Sci. 35:289-298.
  21. Melton, S. L., M. Amiri, G. W. Davis and W. R. Backus. 1982. Flavor and chemical characteristics of ground beef from grass-, forage-, grain- and grain-finished steers. J. Anim. Sci. 55:77-87.
  22. Milanesi, E., L. Nicoloso and P. Crepaldi. 2008. Stearoyl-CoA desaturase (SCD) gene polymorphism in Italian cattle breeds. J. Anim. Breed. Genet. 125(1):63-67.
  23. Monson, F., C. Sanudo and I. Sierra. 2005. Influence of breed and ageing time on sensory meat quality and consumer acceptability in intensively reared beef. Meat Sci. 71:471-479.
  24. Ohsaki, H., A. Thnaka, S. Hoashi, S. Sasazaki, K. Oyama, M. Taniguchi, F. Mukai and H. Mannen. 2009. Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds. Anim. Sci. J. 80:225-232.
  25. Oka, A., F. Iwaki, T. Dohgo, S. Ohtagaki, M. Noda, T. Shiozaki, O. Endoh and M. Ozaki. 2002. Genetic effects on fatty acid composition of carcass fat of Japanese Black Wagyu steers. J. Anim. Sci. 80:1005-1011.
  26. Qin, Z. S., T. Niu and J. S. Liu. 2002. Partition-ligation-expect ation maximization algorithm for haplotype inference with single nucleotide polymorphism. Am. J. Hum. Genet. 71: 1242-1247.
  27. Robins, K., J. Jense, K. J. Ryan, C. Homco-Ryan, F. K. McKeith and M. S. Brewer. 2003. Consumer attitude towards beef and acceptability of enhanced beef. Meat Sci. 65:721-729.
  28. Scheet, P. and M. Stephens. 2006. A fast and flexible statistical model for large-scale population genotype data : Applications to inferring missing genotypes and haplotypic phase. Am. J. Hum. Genet. 78:629-644.
  29. Scollan, N., J. F. Hocquette, K. Nuernberg, D. Dannenberger and I. M. A. Richardson. 2006. Innovation in beef production system that enhance the nutritional and health value of beef lipids and their relationship with meat quality. Meat Sci. 74(1):17-33.
  30. Shin, S. C. 2006. The development of DNA markers related to carcass and meat quality traits in Korean cattle. MS thesis, Sang Ji University.
  31. Smith, S. B., A. G. Clare, K. L. David and A. B. Matthew. 2009. Regulation fat and fatty acid composition in beef cattle. J. Anim. Sci. 22(9):1225-1233.
  32. Sturdivant, C. A., D. K. Lunt, G. C. Smith and S. B. Smith. 1992. Fatty acid composition of subcutaneous and intramuscular adipose tissue and M. longissimus dorsi of Waygu cattle. Meat Sci. 32:449-458.
  33. Taniguchi, M., T. Utsugi, K. Oyama, H. Mannen, M. Kobayashi, Y. Tanabe, A. Ogino and S. Tsuji. 2004. Genotype of stearoyl- CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Anim. Genet. 14:142-148.
  34. Tsuji, S. 2008. Kobe beef as international brand. Symposium of Gyeungbuk Hanwoo Cluster.
  35. Van der Wal, P. G., B. Engel and B. Hulsegge. 1997. Causes for variation in pork quality. Meat Sci. 46(4):319-322.
  36. Vreeland, W. N., R. J. Meagher and A. E. Barron. 2002. Multiplexed, highthroughput genotype by single base extension and endlabeled free_solution electrophoresis. Anal. Chem. 74(17):4328-4333.
  37. Waldman, R. C., G. G. Suess, R. W. Lewis, R. W. Bray and V. H. Brungardt. 1965. Certain fatty acids of bovine tissue and their association with carcass characteristics. J. Anim. Sci. 24:869.
  38. Webb, E. C. and H. A. O'Neil. 2008. The animal fat paradox and meat quality. Meat Sci. 80(1):208-236.
  39. Yosimura, T. and K. Namikawa. 1983. Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine subcutaneous fat. Jpn. J. Zootech. Sci. 54:97-100.
  40. Zembayashi, M., K. Nishimura, D. K. Lunt and S. B. Smith. 1995. Effect of breed type and sex on the fatty acid composition of subcutaneous and intramuscular lipids of finishing steers and heifers. J. Anim. Sci. 73:3325-3332.
  41. Zhang, S., T. J. Knight, J. M. Reecy and D. C. Beitz. 2008. DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim. Genet. 39:62-70.

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