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Epimers/Metabolites of Tetracycline Derivatives; Biological Activity and Regulation Aspects for MRL in Food

생물학적활성을 기초로 한 테트라싸이클린계 항생물질 잔류스크리닝법의 개선과 식품 중 잔류허용기준 설정 개선

  • Kwon, Jin-Wook (National Veterinary Research & Quarantine Service (NVRQS)) ;
  • Yun, Hyo-In (College of Veterinary Medicine, Chungnam Nat'l Univ.) ;
  • Lee, Kyu-Seung (Department of Applied Biology & Chemistry, College of Agriculture & Life Science, Chungnam Nat'l Univ.)
  • 권진욱 (국립수의과학검역원) ;
  • 윤효인 (충남대학교 수의학과) ;
  • 이규승 (충남대학교 응용생명화학과)
  • Received : 2011.02.23
  • Accepted : 2011.03.22
  • Published : 2011.03.31

Abstract

BACKGROUND: Tetracyclines (TCs) are mainly regulated as parent compounds by bioactivity-based screening methods in food. Especially with respect to antimicrobial residues, their metabolites/epimers are also highly concerning chemicals and traditionally applied microbial detection methods are needed to improve with validation for regulatory control. METHODS AND RESULTS: Detection capability and biological activity of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and their epimers; anhydrotetracycline (ATC), epianhydrotetracycline (EATC), epitetracycline (ETC), 4-epi-chlortetracycline (ECTC), 4-epianydrochlotetra-cycline (EACTC), 4-epioxychlortetracycline (EOTC), were measured by microbial growth inhibition screening method of Korea Food Code. CONCLUSION(S): Limited detection capabilities were found, B. megarerium and B. subtilis showed for TC and CTC, and B. subtilis for OTC. Biological potency of each epimer was also presented against various microorganisms, at the level from 50% to 96%, comparing with parent TCs. It is recommended that more advanced microbial screening methods with validation are needed, and biologically active epimers are to be considered as marker residues for MRL setting of regulatory control purpose.

Keywords

Biological potency;Chlortetracycline;Epimer;Oxytetracycline;Tetracycline

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