Repellent Activity of the Extracts of Acorus Gramineus against Mosquito (Culex pipinens pallens)

석창포 추출물의 모기에 대한 기피활성

  • Lee, Seung-Ho (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Jung, Jin-Kwan (Youngil High School) ;
  • Park, Sang-Hyun (Youngil High School) ;
  • Kim, Mun-Sik (Youngil High School) ;
  • Han, Yeon-Soo (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Seo, Jung-Mi (Gwangju Advanced Institute of Health and Environment) ;
  • Kim, In-Seon (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 이승호 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 정진관 (영일고등학교) ;
  • 박상현 (영일고등학교) ;
  • 김문식 (영일고등학교) ;
  • 한연수 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 서정미 (광주광역시 보건환경연구원) ;
  • 김인선 (전남대학교 농업생명과학대학 친환경농업연구소)
  • Received : 2011.02.08
  • Accepted : 2011.03.16
  • Published : 2011.03.31


BACKGROUND: Acorus gramineus is of medicinal plants that exhibit variable biological activities for human health and against insect pests. The extracts of A. gramineus was examined in an attempt to develop a natural repellent against human disease-mediating Culex pipiens. METHODS AND RESULTS: The roots of A. gramineus dried under dark conditions were homogenized and extracted with ethanol. The extracts were subjected to repellent activity assays against C. pipiens in a hand-made acrylamide box with three accessible rooms. Significantly low number of mosquitos was found in the room previously fumigated with the extracts at 50 mg/L on the filter paper, exhibiting less than 20% of mosquitos tested. More than 50% of mosquitos tested was found in the room without the extracts, but less than 30% was found in the room that released mosquitos. GC/MS analysis detected ${\beta}$-asarone as a main component of the extracts. The commercial asarones (${\alpha}$ and ${\beta}$) showed a repellent activity at 50 mg/L on the filter paper similar to the extracts. CONCLUSION(S): A. gramineus has potential for use as a mosquito repellent since ${\beta}$-asarone, a main component of the plant, exhibited a strong repellent activity against C. pipiens.


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