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Application of Macroporous Diatomaceous Earth Column for Residue Analysis of Insecticide Endosulfan in Herbal Medicines

한약재 중 살충제 Endosulfan의 잔류분석을 위한 Macroporous Diatomaceous Earth 컬럼 적용

  • Hwang, Jeong-In (School of Applied Biosciences, Kyungpook National University) ;
  • Jeon, Young-Hwan (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Hyo-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Ji-Hwan (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Yoon-Jeong (Division of Herbal Medicine Research, Korea Food and Drug Administration) ;
  • Park, Ju-Young (Division of Herbal Medicine Research, Korea Food and Drug Administration) ;
  • Kim, Do-Hoon (Division of Herbal Medicine Research, Korea Food and Drug Administration) ;
  • Kim, Jang-Eok (School of Applied Biosciences, Kyungpook National University)
  • 황정인 (경북대학교 응용생명과학부) ;
  • 전영환 (경북대학교 응용생명과학부) ;
  • 김효영 (경북대학교 응용생명과학부) ;
  • 김지환 (경북대학교 응용생명과학부) ;
  • 이윤정 (식품의약품안전청 생약연구과) ;
  • 박주영 (식품의약품안전청 생약연구과) ;
  • 김도훈 (식품의약품안전청 생약연구과) ;
  • 김장억 (경북대학교 응용생명과학부)
  • Received : 2011.03.02
  • Accepted : 2011.03.21
  • Published : 2011.03.31

Abstract

BACKGROUND: Because dried herbal medicines have many active ingredients, it is not easy to determine the residue amount after extraction, partition and clean up of pesticides from them. Especially, liquid-liquid partition method is consuming many times and solvents. Macroporous diatomaceous earth(MDE) column was used to replace the separatory funnel for liquid-liquid extraction with dichloromethane to analyze the endosulfan and its metabolite. METHODS AND RESULTS: The residue analysis method using MDE column instead of liquid-liquid partition for determining insecticide endosulfan and its metabolite in 4 dried herbal medicines was developed by GC/MS. As a result, the recovery rates of the pesticides in 4 herbal medicines were ranged from 80.3 to 93.5% for ${\alpha}$-endosulfan, from 81.0 to 100.3% for ${\beta}$-endosulfan and from 80.6 to 95.6% for endosulfan sulfate, respectively. The coefficients of variation for triplicate were ranged from 1.1 to 3.4%. CONCLUSION: The improved methods are more ecofriendly, safer, faster and less laborious than conventional method by KFDA.

Keywords

Multiresidue analysis method;MDE column;Herbal medicine;Endosulfan;GC/MS

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