Genetic Diversity of avrBs-like Genes in Three Different Xanthomonas Species Isolated in Korea

  • Oh, Chang-Sik (Microbial Safety Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Seung-Don (Research Coordination Division, RDA) ;
  • Heu, Sung-Gi (Microbial Safety Division, National Academy of Agricultural Science, RDA)
  • Received : 2010.10.19
  • Accepted : 2010.12.15
  • Published : 2011.03.01


Plant-pathogenic bacteria including Xanthomonas spp. carry genetic diversity in composition of avirulence genes for interaction with their host plants. Previously, we reported genetic diversity of avirulence genes in X. axonopodis pv. glycines. In this study, we determined genetic diversity of five avirulence genes, avrBs1, avrBs2, avrBs3, avrBs4, and avrRxv, in three other Xanthomonas species isolated in Korea by genomic southern hybridization. Although Korean races of X. campestris pv. vesicatoria that were isolated from year 1995 to 2002 had the same avirulence gene patterns as those that already reported, there was race shift from race 3 to race 1 by acquisition of avrBs3 genes. X. campestris pv. campestris isolated from Chinese cabbage, but not from cabbage or radish, carried two avrBs3 genes, and one of them affected HR-eliciting ability of this bacterium in broccoli. X. oryzae pv. oryzae carried eight to thirteen avrBs3 gene homologs, and this bacterium showed dynamic changes of resistance patterns in rice probably by losing or obtaining avrBs3 genes. These results indicate that avrBs3 gene is more diverse in Xanthomonas spp. than other four avirulence genes and also host ranges of these bacteria can be easily changed by loss or acquisition of avrBs3 genes.


  1. Alfano, J. R. and Collmer, A. 2004. Type III secretion system effector proteins: double agents in bacterial disease and plant defense. Annu. Rev. Phytopathol. 42:385-414.
  2. Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A. and Bonas, U. 2009. Breaking the code of DNA binding specificity of TAL-tyep III effectors. Science 326:1509-1512.
  3. Bonas, U., Stall, R. E. and Staskawicz, B. 1989. Genetic and structual characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria. Mol. Gen. Genet. 218:127-136.
  4. Ciesiolka, L. D., Hwin, T., Gearlds, J. D., Minsavage, G. V., Saenz, R., Bravo, M., Handley, V., Conover, S. M., Zhang, H., Caporgno, J., Phengrasamy, N. B., Toms, A. O., Stall, R. E. and Whalen, M. C. 1999. Regulation of expression of avirulence gene avrRxv and identification of a family of host interaction factors by sequence analysis of avrBsT. Mol. Plant- Microbe Interact. 12:35-44.
  5. Gurlebeck, D., Thieme, F. and Bonas, U. 2006. Type III effector proteins from the plant pathogen Xanthomonas and their role in the interaction with the host plant. J. Plant Physiol. 163:233-255.
  6. Hopkins, C. M., White, F. F., Choi, S.-H., Guo, A. and Leach, J. E. 1992. Identification of a family of avirulence genes from Xanthomonas oryzae pv. oryzae. Mol. Plant-Microbe Interact. 5:451-459.
  7. Huang, N., Angeles, E. R., Domingo, J., Magpantay, G., Singh, S. Z. G., Kumaravadivel, N., Bennett, J. and Khush, G. S. 1997. Pyramiding of bacterial blight resistance genes in rice: markerassisted selection using RFLP and PCR. Theor. Appl. Genet. 95:313-320.
  8. Jones, J. B. and Stall, R. E. 1998. Diversity among Xanthomonads pathogenic on pepper and tomato. Annu. Rev. Phytopathol. 36:41-58.
  9. Kay, S. and Bonas, U. 2009. How Xanthomonas tyep III effectors manipulate the host plant. Curr. Opin. Microbiol. 12:37-43.
  10. Leach, J. E., Vera Cruz, C. M., Bai, J. and Leung, H. 2001. Pathogen fitness penalty as a predictor of durability of disease resistance genes. Ann. Rev. Phytopathol. 39:187-224.
  11. Moscou, M. and Bogdanove, A. J. 2009. A simple cipher governs DNA recognition by TAL effectors. Science 326:1501.
  12. Nino-Liu, D. O., Ronald, P. C. and Bogdanove, A. J. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol. Plant. Pathol. 7:303-324.
  13. Park, H.-J., Han, S.-W., Oh, C.-S., Lee, S., Ra, D., Lee, S.-H. and Heu, S. 2008. Avirulence gene diversity of Xanthomonas axonopodis pv. glycines isolated in Korea. J. Microbiol. Biotechnol. 18:1500-1509.
  14. Schornack, S., Ballvora, A., Gurlebeck, D., Peart, J., Genal, M., Baker, B., Bonas, U. and Lahaye, T. 2004. The tomato resistance protein Bs4 is a predicted non-nuclear TIR-NB-LRR protein that mediates defense responses to severely truncated derivatives of AvrBs4 and overexpressed AvrBs3. Plant J. 37:46-60.
  15. Schornack, S., Meyer, A., Romer, P., Jordan, T. and Lahaye, T. 2006. Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins. J. Plant Physiol. 163:256-272.
  16. Swords, K. M. M., Dahlbeck, D., Kearney, B., Roy, M. and Staskawicz, B. J. 1996. Spontaneous and induced mutations in a single open reading frame alter both virulence and avirulence in Xanthomonas campestris pv. vesicatoria avrBs2. J. Bacteriol. 178:4661-4669.
  17. Van den Ackerveken, G., Marois, E. and Bonas, U. 1996. Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell 87:1307-1316.
  18. Yang, B. and White, F. F. 2004. Diverse members of the AvrBs3/ PthA family of type III effectors are major virulence determinants in bacterial blight disease of rice. Mol. Plant-Microbe Interact. 17:1192-1200.
  19. Yang, B., Zhu, W., Johnson, L. B. and White, F. F. 2000. The virulence factor AvrXa7 of Xanthomonas oryzae pv. oryzae is a type III secretion pathway-dependent nuclear-localized double- stranded DNA-binding protein. Proc. Natl. Acad. Sci. USA 97:9807-9812.

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