The Effect of Anti-atopic Cosmetic in Hairless Mice

항 아토피 화장품이 아토피 동물모델 Hairless Mice에 미치는 영향

  • Kwon, Taek Kwan (Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Lim, Kun Bin (FineCo Ltd., Bio Venture Plaza) ;
  • Kim, Jin-Chul (Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 권택관 (강원대학교 바이오산업공학부 생물소재공학전공) ;
  • 임건빈 (화인코주식회사) ;
  • 김진철 (강원대학교 바이오산업공학부 생물소재공학전공)
  • Received : 2010.11.08
  • Accepted : 2010.11.24
  • Published : 2011.02.10

Abstract

The efficacies of anti-atopic preparations were investigated in hairless mice suffering from 2,4-dinitro-chlorobenzene (DNCB)-induced atopic dermatitis-like lesion. Solid lipid nanoparticle (SLN) containing ceramide and astaxanthin was prepared by a melt-homogenization method using Aminsoft-CT 12 (Cocoyl glutamate) as an emulsifier. And then, the SLNs were coated with silk fibroin (SF) by taking advantage of an electrostatic interaction between the surface of SLNs and SF. SLNs were included in lotion (FL) and cream (FC) types of preparations. Anti-atopic efficacies of the preparations were investigated in terms of appearance of skin surface, spleen index, serum IgE level, and serum cytokine level. SLN-containing preparations suppressed IgE production and IL-4 expression, but promoted $IFN-{\gamma}$ expression.

Acknowledgement

Supported by : 지식경제부

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