Enzyme-linked Immunosorbent Assay Strip Sensor for Rapid Detection of Staphylococcus aureus

Staphylococcus aureus 신속 검출을 위한 효소면역측정 스트립 센서

  • Park, So Jung (Department of Chemical Engineering, Hankyong National University) ;
  • Kim, Young-Kee (Department of Chemical Engineering, Hankyong National University)
  • 박소정 (한경대학교 화학공학부) ;
  • 김영기 (한경대학교 화학공학부)
  • Received : 2011.07.29
  • Accepted : 2011.09.21
  • Published : 2011.10.10

Abstract

In this study, an established enzyme-linked immunosorbent assay and immuno-chromatography technique are combined to fabricate an immuno-strip sensor for the detection of S. aureus. The immuno-strip is manufactured by using four different functional membranes. The capture antibody is immobilized on the nitrocellulose membrane due to the high affinity and the capillary action through porous membranes induces a flow of sample. A colorimetric signal is appeared according to the enzyme reaction and is analyzed by the digital camera (qualitative analysis) and home-made image analysis software (quantitative analysis). Under the optimal conditions, samples with S. aureus in the range of $2.7{\times}10^4{\sim}2.7{\times}10^7CFU/mL$ can be detected by the colorimetric method within 30 min.

Keywords

Staphylococcus aureus;immuno-strip sensor;enzyme-linked immunosorbent assay (ELISA);immuno-chromatographic assay

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