Suppression Effects of Sopunghwalhyeol-tang(Shufenghuoxie-tang) on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

소풍활혈탕(疎風活血湯)이 Monosodium Iodoacetate로 유발한 흰쥐의 골관절염 억제에 미치는 영향

  • Kim, Dae-Hyoung (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Jeong, Su-Hyeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Soon-Joong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University)
  • 김대형 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 정수현 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.12.13
  • Accepted : 2011.01.04
  • Published : 2011.01.30

Abstract

Objectives : This study was to investigate the suppression effects of Sopunghwalhyeol-tang(Shufenghuoxie-tang) on the monosodium iodoacetate-induced osteoarthritis in rats. Methods : Arthritis was induced by injection of monosodium iodoacetate(0.5 mg) into the both knee joints of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken distilled water for 20 days. Treated group was taken extracts of Sopunghwalhyeol-tang(Shufenghuoxie-tang) by oraly for same duration. Normal group(n=8) was injected with normal saline and was taken distilled water for 20 days. Macroscopic examination and histopathological study on articular cartilage of knee joint were operated at 20 days after injection. Proteoglycan(PG) content of articular cartilages of knee joint was represented by safranine O staining, was measured at 20 days after injection. Tumor necrosis $factor-{\alpha}(TNF-{\alpha})$, $interleukin-1{\beta}(IL-1{\beta})$, in synovial fluid were measured with enzyme-linked immuno sorbent assay(ELISA) kit at 20 days after injection. Immunohistochemical staining of cyclo-oxygenase-2(COX-2), inducible nitric oxide synthase(iNOS) in knee joints were observed at 20 days after injection. Results : 1. Lymphocytes in peripheral blood the treated group was significantly decreased compared with the control group. 2. PG content in articular cartilage of the treated group was significantly increased compared with the control group. 3. Histopathologically, osteoarthritic scores of the treated group was significantly decreased compared with the control group. 4. $TNF-{\alpha}$ content in synovial fluid of the treated group was significantly decreased compared with the control group. 5. COX-2 revelation index in chondrocytes and synovial membrane of the treated group was significantly decreased compared with the control group. 6. Matrix metalloproteinase-3(MMP-3) revelation index in chondrocytes and synovial membrane of the treated group was significantly decreased compared with the control group. Conclusions : On the basis of these results, we concluded that Sopunghwalhyeol-tang(Shufenghuoxie-tang) has inhibiting effects on the $TNF-{\alpha}$, COX-2 and MMP-3 secretion of chondrocytes and synovial membrane in Monosodium Iodoacetate-induced osteoarthritis model of rats.

Keywords

Monosodium iodoacetate;Sopunghwalhyeol-tang(Shufenghuoxie-tang);Proteoglycan;$TNF-{\alpha}$;COX-2;MMP-3

Acknowledgement

Supported by : 지식경제부

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