Inflammatory Effect of Rheum undulatum L.

대황추출물의 염증반응 제어효과

  • Jeun, Dong-Joo (Dept. of Pharmaceutical Engineering, College of Health Science, Sang-Ji University) ;
  • Cha, Yun-Yeop (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sang-Ji University) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of Health Science, Sang-Ji University)
  • 전동주 (상지대학교 보건과학대학 제약공학과) ;
  • 차윤엽 (상지대학교 한의과대학 한방재활의학과교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과)
  • Received : 2010.10.27
  • Accepted : 2010.11.18
  • Published : 2011.01.30

Abstract

Objectives : The present study investigated inflammatory effect of Rheum undulatum L. in lipopolysaccharide-exposed rats and Raw 264.7 cells. Methods : Male rats weighting $185.39{\pm}8.21g$ fed basal diet for 1 week and 32 rats were divided into a control group and 3 experimental groups. We fed a control group of rats a basal diet and administered normal saline(100 mg/kg, 1time/1day) for 6 weeks. And we fed basal diet and administered an extract of Rheum undulatum L.(100 mg/kg, 200 mg/kg, 300 mg/kg, 1time/1day) to each experimental group of rats. We measured the plasma concentration of $IL-1{\beta}$($interleukin-1{\beta}$), IL-6 and $TNF-{\alpha}$(tumor necrosis $factor-{\alpha}$), liver cytokines, Raw 264.7 macrophages cytokines. Results : The plasma concentration of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ peaked at 5h(hour) after LPS(lipopolysaccharides) injection, and the values of the Rheum undulatum L. extract groups were lower than those of the control group. In the increment of these cytokines concentration at 2h and 5h after LPS injection, the Rheum undulatum L. groups were lower than that of control group. The plasma concentration of IL-10 peaked at 5h after LPS injection, and the values of the Rheum undulatum L. extract groups were higher than those of the control group. In the increment of this cytokine concentration at 2h and 5h after LPS injection, the Rheum undulatum L. groups were higher than that of control group. Liver cytokines measurement was done at 5h after LPS injection. The concentration of liver $IL-1{\beta}$ and IL-6 in the Rheum undulatum L. groups was lower than that of the control group. The concentrations of liver $TNF-{\alpha}$, and IL-10 showed no significant differences among all the treatment groups. In the studies of lipopolysaccharide-exposed Raw 264.7 cells, the concentration of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ in the lipopolysaccharide-exposed cells groups was higher than that of control group(normal group), and in the lipopolysaccharide-exposed cells groups, these values showed a tendency to decrease in the Rheum undulatum L. groups. The concentration of IL-10 in the lipopolysaccharide-exposed cells groups was higher than that of control group(normal group), and in the lipopolysaccharide-exposed cells groups, the values showed a tendency to increase in the Rheum undulatum L. groups. Conclusions : These results indicate that the Rheum undulatum L. extracts have an functional material for inflammatory activities.

Keywords

Inflammatory effect;Rheum undulatum L;LPS shock;cytokines;Raw 264.7 cells

References

  1. Chao CY, SL Yeh, MT Lin, WJ Chen. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids and inflammatory mediators in septie rats. Nutrition. 2000;16:284-8. https://doi.org/10.1016/S0899-9007(99)00299-3
  2. Rabson A, IM Roitt, PJ Delves. Really essential medical immunology. Blackwell publishing Ltd, Oxford. 2005:1-14.
  3. Barton CC, EX barton, PE Ganey, SL Kunkel, RA Roth. Bacterial lipopolysaccharide enhances aflatoxin $B_1$ hepatotoxicity in rats by a mechanism that depends on tumor necrosis factor-${\alpha}$. Hepatology. 2001;33:66-73. https://doi.org/10.1053/jhep.2001.20643
  4. Seo WG, Pae HO, Oh GS, KY Chai, Kwon TO, Yun YG, Kim NY, Chung HT. Inhibitory rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, Raw 264.7 cells. J Ethnopharmacol. 2001;76:59-64. https://doi.org/10.1016/S0378-8741(01)00221-5
  5. Surh YJ. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxieol. 2002;40(8):1091-7. https://doi.org/10.1016/S0278-6915(02)00037-6
  6. 고인자, 유승조, 이은방. 한국산 하고초류의 약물학적 연구(1). 생약학회지. 1986;17(3):232-41.
  7. 김태희, 양기숙, 황은진, 박성배. 마황의 면역 작용에 마치는 영향. 생약학회지. 1991;22(3):183-91.
  8. 남정연, 이현선, 이승웅, 정미연, 최정호, 유은숙, 노문철, 김영국. 감국에서 분리한 Kikkanol F Monoacetate와 5-Hydroxy-6,7,3',4'-tetramethoxyflavone의 IL-6생성억제활성. 생약학회지. 2005;36(3):186-90.
  9. 김선재, 박윤미, 정순택. 감국추출물의 항충치 효과와 Glucosyltransferase 저해활성탐색. Korean food culture. 2005;20(3):341-5.
  10. Lee E. Anti-inflammatory effect of Scutellariae Radix. Korean J. Plant Res. 2007;20(6):522-48.
  11. 육창수. 원색한국약용식물도감. 서울:아카데미서적. 1993:158.
  12. 육창수. 한약한 II. 서울:동명사. 1992:206.
  13. 허준., 국역증보동의보감. 서울:남산당. 1981:1204.
  14. 전국한의과대학 본초학교실. 본초학. 서울:영림사. 1991:242-4.
  15. Shah CS, Qardy JS, Bhatt JG. Qualitative and quantitative evaluation of anthraquinone derivatives in Indian rhubarb. Planta Med. 1972;22:103-8. https://doi.org/10.1055/s-0028-1099590
  16. 장연희, 최상원, 조성희. 고지방식이 급여 흰쥐에서 대황 및 대황정제환의 혈청 지질 개선 효과. 한국영양학회지. 2008;41(1):5-12.
  17. 조성희, 박소영, 최상원. 당뇨 생쥐에서 대황 추출물 및 정제환의 혈당과 지질 상태 개선 효과. 한국영양학회지. 2008;41(6):493-501.
  18. 이영종. 대황 전탕액 분획이 고지사료 투여 흰쥐의 혈중 지질 함량에 미치는 영향. 대한본초학회지. 2000;15(2):87-93.
  19. 윤현정, 황성구, 윤형중, 김창현, 서교수, 박원환, 박선동. 간암 세포주 HepG2에 대한 대황 추출물의 항암효과. 대한본초학회지. 2006;21(4):27-36.
  20. 박성주, 정종길, 서상완, 황상욱, 김영우, 송달수, 채영석, 신민교, 송호준. CCK로 유발시킨 급성 훼장염에 대한 가미대황목단피당의 효과. 대한본초학회지. 2005;20(3):59-65.
  21. Marriot JB, M Westby, S Cookson, M Guckian, S Goodbourn, G Muller. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-${\alpha}$ production. J Immunol. 1998;161:4236-43.
  22. Mathiak G, G Grass, T Herzmann, T Luebke, C Cu-Zetina, SA Boehm. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-Iethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000;131:383-6. https://doi.org/10.1038/sj.bjp.0703629
  23. Eduard FM, Martha SMR, Victor PA, Pablo M. Immunomodulatory effects of thalidomide analogs on LPS-induced plasma and hepatic cytokines in the rat. Biochemical pharmacology. 2004;68:1321-9. https://doi.org/10.1016/j.bcp.2004.06.018
  24. Aono K, K Isobe, K Kuichi, Z Fan, M Ito, A Takeuchi. In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. J cell Biochem. 1997;65:349-58. https://doi.org/10.1002/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S
  25. Corral LG, GW Muller, AL Moreira, X Chen, M Wu, D Stirling. Selection of novel analogs of thalidomide with enhanced tumor necrosis factor-${\alpha}$ inhibitory activity. Mol med. 1996; 25:964-9.
  26. Sang H, GL Wallis, CA Stewart, K Yashige. Expression of cytokines and activation of transcription factors in lipopolysaccharide-administered rats and their inhibition by phenyl N-tert-butylnitrone(PBN). Arch Biochem Biophys. 1999;363:341-8. https://doi.org/10.1006/abbi.1998.1086
  27. Harry D, R Anand, S Holt, S Davies, R Marley, B Fernando. Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic rat. Hepatology. 1999;30:1198-205. https://doi.org/10.1002/hep.510300515
  28. Chamulitrat W, ME Blazka, SJ Jordan, MI Luster, RP Mason. Tumor necrosis factor-${\alpha}$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995;24:2273-80.
  29. Harbrecht BG, M DiSilvio, AJ Demetris, RL Simmons, TR Billiar. Tumor necrosis factor--${\alpha}$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994;20:1055-60. https://doi.org/10.1002/hep.1840200439
  30. Hamada E, T Nishida, Y Uchiyama, J Nakamura, K Isihara, H Kazuo. Activation of Kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J Hepatol. 1999;30:807-18. https://doi.org/10.1016/S0168-8278(99)80133-0
  31. Thompson KC, A Trowern, A Fowell, M Marathe, C Haycock, MJP Arthur et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998;28:1518-24. https://doi.org/10.1002/hep.510280611
  32. Moreira AL, EP Sampaio, A Zmuidzinas, P Frindt, KA Smith, G Kaplan. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J Exp Med. 1993;177:1675-80. https://doi.org/10.1084/jem.177.6.1675
  33. Simpson KJ, Lukacs NW, Colletti L, Strieter RM, Kunkel SL, Cytokines and the liver. J Hepatol. 1997;27:1120-32. https://doi.org/10.1016/S0168-8278(97)80160-2
  34. Beutler B, Greenwald B, Hulmes JD, Chang M, Pan YC, Maathison J et al. Identity of tumor necrosis factor and the macrophage secreted factor cachectin. Nature. 1985;316:552-6. https://doi.org/10.1038/316552a0
  35. Klapproth J, Geiger T, Andus T, Heirich PC. Fate and biological action of human recombinant interleukin 1${\beta}$ in the rat in vivo. Eur J Immunol. 1980;19:1485-90.
  36. Louis H, LeMoine O, Peny MO, Quertinmont E, Fokan D, Goldman M et al. Production and role of interleukin-10 in concanavalin A-induced hepatitis in mice. Hepatology. 1997;25:1382-9. https://doi.org/10.1002/hep.510250614