Effect of Artemisia Capillaris Thunberg EtOH Ext. on Lowering Lipid, Anti-oxidation and Concentration of Plasma Inflammatory Mediators Using Rats Fed on High-oxidized Fat

인진호(茵蔯蒿) 추출물이 과산화지질 투여한 쥐의 지질강하, 항산화효과 및 염증매개물질의 생산에 미치는 영향

  • Kong, In-Pyo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sang-Ji University) ;
  • Lee, Eun (Dept of Pharmaceutical Engineering, College of Health Science, Sang-Ji University) ;
  • Cha, Yun-Yeop (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sang-Ji University)
  • 공인표 (상지대학교 한의과대학 한방재활의학과교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 차윤엽 (상지대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.11.29
  • Accepted : 2010.12.27
  • Published : 2011.01.30

Abstract

Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.

Keywords

Artemisia capillaris thunberg;Anti-oxidation;Plasma inflammatory mediators

Acknowledgement

Supported by : 상지대학교

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