Effect of Storage in Different Commercial Semen Extenders on the Motility, Viability and Fertility In Vitro of Boar Spermatozoa

수퇘지 정자의 운동성, 생존성 및 체외수정 능력에 대한 시판 액상 정액 보존액과 보존 기간의 영향

  • 사수진 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김명직 (농촌진흥청 국립축산과학원 양돈과) ;
  • 조규호 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김두완 (농촌진흥청 국립축산과학원 양돈과) ;
  • 소경민 (농촌진흥청 국립축산과학원 양돈과) ;
  • 정기화 (경남과학기술대학교 동물소재공학과) ;
  • 손중호 (노아바이오텍) ;
  • 김인철 (농촌진흥청 국립축산과학원 양돈과)
  • Received : 2011.08.17
  • Accepted : 2011.08.24
  • Published : 2011.09.30

Abstract

The objective of this study was to determine the effect of semen extenders on the motility, viability and fertility in vitro of spermatozoa during storage of fresh boar semen diluted in different commercial extenders used for pig artificial insemination (AI). In this experiment, semen were diluted in Androhep plus, Beltsville Thawing Solution (BTS), Modena, Seminark and Vitasem LD. Five ejaculates were collected from three Duroc boars and sub-samples were diluted ($30{\times}10^6$ spermatozoa/ml) in different extenders. Semen was stored at $170^{\circ}C$ for 10 days. Sperm motility and viability was assessed using Computer-Assisted Semen Analysis (CASA) and flow-cytometry on 1, 3, 5 and 10 day post collection The motility of spermatozoa stored in different extenders was gradually decreased by increasing the duration of storage of semen. However, there was not significant1y different in the sperm motility and viability among other extenders. On the other hand, the in vitro-matured oocytes were fertilized and cultured in vitro to assess the fertility of boar spermatozoa stored for 3 and 10 days in different extenders. The percentage of morula and blastocyst were taken as indicators of fertility in vitro of spermatozoa. Therefore, there were no differences in the rate of embryos developed to the molular and blastocyst stage. There were no differences in the motility and fertility in vitro among 5 kinds of commercial boar semen extenders.

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