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벼 키다리병 저항성 검정을 위한 기내 유묘 검정법 개발

Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease

  • 이용환 (농촌진흥청 기획조정관실) ;
  • 이명지 (국립농업과학원 농업생물부) ;
  • 최효원 (국립농업과학원 농업생물부) ;
  • 김성택 (국립농업과학원 농업생물부) ;
  • 박진우 (국립농업과학원 농업생물부) ;
  • 명인식 (국립농업과학원 농업생물부) ;
  • 박경석 (국립농업과학원 농업생물부) ;
  • 이세원 (국립농업과학원 농업생물부)
  • Lee, Yong-Hwan (Knowledge and Information Officer Division, Rural Development Administration) ;
  • Lee, Myeong-Ji (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Choi, Hyo-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Kim, Sung-Taek (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Jin-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Myung, Inn-Shik (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Kyung-Seok (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Lee, Se-Weon (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration)
  • 투고 : 2011.11.16
  • 심사 : 2011.11.28
  • 발행 : 2011.12.31

초록

벼 키다리병은 매우 중요한 벼의 병 가운데 하나로 아시아 지역에서 넓게 발생하고 있다. 포장에서의 저항성 검정체계가 개발되어 있지만, 그 검정 결과가 환경조건에 따라 다르게 나타나는 문제점을 가지고 있다. 본 연구에서는 정확하고 빠른 키다리병 품종 저항성 검정을 위하여 기내에서 검정하는 시스템을 개발하였다. 병원균을 접종한 '남평벼', '주남벼', '추청벼', '삼천벼', '오대벼', '화신벼' 등 6개 품종의 종자를 MS배지가 10 ml 씩 담겨 있는 직경 30 mm, 길이 450 mm 시험관에 벼 종자 3립씩 파종 후 $25^{\circ}C$$28^{\circ}C$ 항온기에 치상하고 하루 12시간 광조건으로 배양하였다. 키다리병 증상은 벼가 도장한 후에 2-3주 경과시점 부터 잎 끝이 마르기 시작하여 고사하게 된다. $10^5$ 포자/ml 농도와 $28^{\circ}C$ 조건에서 '남평벼'는 파종후 28일째부터 잎 끝이 마르는 증상을 보이기 시작하여 35일째에 완전히 고사를 하는 반면 다른 품종들은 19-23일째에 모두 고사하였다. 기내유묘검정법을 이용하여 72개 품종에 대해 저항성검정을 한 결과 '남평벼'와 '인월벼'를 저항성으로 '화동벼', '석정벼', '삼광벼', '삼평벼', '농호벼', '흑진주벼', '조안벼'를 중도저항성으로 선발하였다. 기내유묘검정법을 이용할 경우 파종 후 35일 이내에 키다리병 품종저항성 검정을 완료할 수 있었다.

키워드

Fusarium fujikuroi;Rice;Resistance;Cultivar

과제정보

연구 과제번호 : Research Program for Agricultural Science & Technology Development

연구 과제 주관 기관 : National Academy of Agricultural Science, Rural Development Administration

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  2. Mapping of qBK1, a major QTL for bakanae disease resistance in rice vol.35, pp.2, 2015, https://doi.org/10.1007/s11032-015-0281-x
  3. Large-scale screening of rice accessions to evaluate resistance to bakanae disease vol.80, pp.5, 2014, https://doi.org/10.1007/s10327-014-0528-0
  4. Bakanae Disease Reduction Effect by Use of Silicate Coated Seed in Wet Direct-Seeded Rice vol.61, pp.1, 2016, https://doi.org/10.7740/kjcs.2016.61.1.009
  5. Identification of bakanae disease resistance loci in japonica rice through genome wide association study vol.10, pp.1, 2017, https://doi.org/10.1186/s12284-017-0168-z
  6. Molecular mapping of qBK1WD, a major QTL for bakanae disease resistance in rice vol.11, pp.1, 2018, https://doi.org/10.1186/s12284-017-0197-7
  7. Mapping of a major quantitative trait locus for bakanae disease resistance in rice by genome resequencing vol.293, pp.3, 2018, https://doi.org/10.1007/s00438-017-1407-0