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Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease

벼 키다리병 저항성 검정을 위한 기내 유묘 검정법 개발

  • Lee, Yong-Hwan (Knowledge and Information Officer Division, Rural Development Administration) ;
  • Lee, Myeong-Ji (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Choi, Hyo-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Kim, Sung-Taek (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Jin-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Myung, Inn-Shik (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Kyung-Seok (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Lee, Se-Weon (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration)
  • 이용환 (농촌진흥청 기획조정관실) ;
  • 이명지 (국립농업과학원 농업생물부) ;
  • 최효원 (국립농업과학원 농업생물부) ;
  • 김성택 (국립농업과학원 농업생물부) ;
  • 박진우 (국립농업과학원 농업생물부) ;
  • 명인식 (국립농업과학원 농업생물부) ;
  • 박경석 (국립농업과학원 농업생물부) ;
  • 이세원 (국립농업과학원 농업생물부)
  • Received : 2011.11.16
  • Accepted : 2011.11.28
  • Published : 2011.12.31

Abstract

Rice bakanae disease, caused by the fungus Fusarium fujikuroi, is one of the most important rice diseases and distributed widely in Asia. Resistance screening system in rice field had been established. However, the evaluation results of the system vary according to the environmental conditions when the test is conducted. To develop precise and rapid evaluation method of disease resistance of rice to bakanae disease, in vitro screening system was attempted in this study. The six cultivars namely, 'Nampyeongbyeo', 'Junambyeo', 'Chucheongbyeo', 'Samcheonbyeo', 'Odaebyeo' and 'Hwasinbyeo' were tested. They were planted onto MS agar medium (10 ml) in test tube ($450\;mm{\times}{\phi}30\;mm$) and incubated at $25^{\circ}C$ and $28^{\circ}C$ in growth chamber under 12 hr light condition. Symptoms of over growth appeared a few days after seeding and then seedling were withered 2-3 weeks after over growth. The disease symptoms such as leaf dryness on top of rice were appeared in the 'Nampyeongbyeo' from 28 days at the concentration of $10^5$ spores/ml culturing at $28^{\circ}C$ and then withered completely 35 days after seeding. Whereas the other varieties withered entirely 19-23 days after seeding. Using the in vitro seedling screening method, 72 rice varieties were investigated to select resistant cultivar. Finally, two resistant cultivars ('Nampyeongbyeo' and 'Inwolbyeo') and seven moderately resistant cultivars ('Hwadongbyeo', 'Seokjeongbyeo', 'Samgwangbyeo', 'Sampyeongbyeo', 'Nonghobyeo', 'Heukjinjubyeo' and, 'Joanbyeo') were selected. If in vitro seedling screening method was used for evaluation of bakanae disease resistance, it would be completed within 35 days after sowing of rice seed.

Keywords

Fusarium fujikuroi;Rice;Resistance;Cultivar

Acknowledgement

Grant : Research Program for Agricultural Science & Technology Development

Supported by : National Academy of Agricultural Science, Rural Development Administration

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