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Anti-inflammatory and PPAR Subtypes Transactivational Activities of Phenolics and Lignans from the Stem Bark of Kalopanax pictus

  • Quang, Tran Hong ;
  • Ngan, Nguyen Thi Thanh ;
  • Minh, Chau Van ;
  • Kiem, Phan Van ;
  • Nhiem, Nguyen Xuan ;
  • Tai, Bui Huu ;
  • Thao, Nguyen Phuong ;
  • Luyen, Bui Thi Thuy ;
  • Song, Seok-Bean ;
  • Kim, Young-Ho
  • Received : 2011.08.25
  • Accepted : 2011.09.26
  • Published : 2011.11.20

Abstract

A new compound, kalopanaxin F (3), and 11 known compounds (1, 2, 4-12), were isolated from the stem bark of Kalopanax pictus. Their structures were elucidated on the basis of chemical and spectroscopic methods. Five of the compounds (2, 3, 5, 6, and 12) significantly inhibited $TNF{\alpha}$-induced NF-${\kappa}B$ transcriptional activity in HepG2 cells in a dose-dependent manner, with $IC_{50}$ values ranging from 6.2 to 9.1 ${\mu}M$. Furthermore, the transcriptional inhibitory function of these compounds was confirmed based on decreases in COX-2 and iNOS gene expression in HepG2 cells. Compounds 3-7, 9, and 12 significantly activated the transcriptional activity of PPARs dose-dependently, with $EC_{50}$ values ranging from 4.1-$12.7{\mu}M$. Compounds 4 and 5 exhibited $PPAR{\alpha}$, $PPAR{\gamma}$, and $PPAR{\beta}({\delta})$ transactivational activities in a dose-dependent manner, with $EC_{50}$ values of 16.0 and 17.0, 8.7 and 16.5, 26.2 and 26.3 ${\mu}M$, respectively.

Keywords

Kalopanax pictus;Araliaceae;Phenolic;Anti-inflammatory;PPAR transactivational activity

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Cited by

  1. Comparison of Phenolic Acid from Shoots of Aralia elata and Kalopanax pictus Cultivated in Korea Using UPLC-DAD-ESI(+)-QToF/MS vol.37, pp.4, 2018, https://doi.org/10.5338/KJEA.2018.37.4.37

Acknowledgement

Supported by : Korea Food and Drug Administration, National Research Foundation of Korea (NRF)