Inhibitory Effect of Rhus Verniciflua Extract on Lipid Peroxidation and Inflammatory Cytokines during Endurance Exercise Training

지구성 운동시 옻나무 추출액의 지질과산화 및 염증성사이토카인 억제효과

  • Lee, Youn-Kyung (Dept. of Physical Education, Kyungpook National University) ;
  • Kwon, Oh-Seon (Dept. of Physical Education, Kyungpook National University) ;
  • Song, Young-Ju (Division of Sports Science, Sunmoon University) ;
  • Kim, Sea-Hyun (Tree Breeding Division, Korea Forest Research Institute) ;
  • Kim, Pan-Gi (School of Ecological & Environmental Systems, Kyungpook National University) ;
  • Ryu, Sung-Pil (Dept. of Leisure Sports, Kyungpook National University)
  • 이윤경 (경북대 체육교육과) ;
  • 권오선 (경북대 체육교육과) ;
  • 송영주 (선문대학교 스포츠과학부) ;
  • 김세현 (국립산림과학원 특용자원연구과) ;
  • 김판기 (경북대 생태환경시스템학부) ;
  • 류승필 (경북대 레저스포츠학과)
  • Received : 2010.10.12
  • Accepted : 2010.02.11
  • Published : 2010.03.31

Abstract

This study was performed to find out the inhibitory effect of Rhus Verniciflua extract on lipid peroxidation and inflammatory cytokines during endurance exercise training for 8 weeks in rats. For this study, Sprague-Dawley rats were divided into 4 groups; sedentary (SED), exercise training (TRA), RVS extract ingestion (RVE), and RVS extract ingestion and exercise training (RVE-TRA). TRA and RVE-TRA were trained on treadmill with increasing speed gradually and administered 10 mL/kg/d of Rhus Verniciflua extract orally to RVE and RVE-TRA. In order to analyze antioxidant function, blood SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), and MDA (malondialdehyde) were examined. And, analysis of inflammatory cytokines were examined using IL-6 (interleukin-6), TNF-${\alpha}$ (tumor necrosis factor-alpha), CRP (C-reactive protein), and NO (nitric oxide). SOD in TRA was significantly higher than SED and RVE (p<0.05), and RVE-TRA was highest among the groups (p<0.05). The MDA content of TRA, RVE and RVE-TRA were significantly lower than SED. GSH-Px activity of SED was significantly lower than other groups (p<0.05). IL-6 and TNF-${\alpha}$ content of RVE and RVE-TRA were significantly lower than SED and TRA (p<0.05). CRP concentration of SED was the lowest among groups (p<0.05). Finally, NO concentration of SED and TRA were higher than RVE and RVE-TRA (p<0.05). These results suggested that it is efficient for rats to reduce lipid peroxidation and induce anti-inflammatory by taking RVS extract during exercise training. Afterwards, if studies on the properties of RVS extract can be made with various ways, use of Rhus Verniciflua trees might be made widely which are growing naturally in mountains in Korea.

References

  1. 김인원, 신동화, 백남인. 1999a. 옻나무 에탄올 추출물로부터 항산화 활성 물질의 구조동정. 한국식품과학회지 31(6): 1654-1660.
  2. 김인원, 신동화, 최웅. 1999b. 한약재로부터 선발된 옻나무 수피 추출물로부터 항산화 활성물질의 분리. 한국식품과학회지 31(3): 855-863.
  3. 박희준, 이경태, 박건영, 한갑이, 정민화, 최종원. 2002. 간섬유화 동물에서 옻나무 목부로부터 분리한 flavonoids의 독성 경감기전. 생명과학회지 12(3): 332-339.
  4. 류승필, 이수천. 2006. 운동과 항산화제. 한국체육학회지 45(1): 699-706.
  5. 이윤경, 이수천, 전병덕, 김세현, 김판기, 류승필. 2009. 옻나무 추출액 섭취와 운동훈련이 흰쥐의 혈중 지질 및 인슐린 저항성에 미치는 영향. 한국임학회지 98(6): 인쇄중
  6. 이효승, 허숙경, 윤현정, 최재우, 정재하, 박선동. 2008. 마우스 대식세포에서 血竭(Draconis Resina)의 항산화 및 항염증 효과. 한국본초학회지 23(2): 179-192.
  7. 임계택, 심재환. 1997. 옻나무 에탄올 추출물의 쥐 뇌세포에 대한 항산화효과. 한국식품과학회지 29(6): 1248-1254.
  8. 임계택, 이정채. 1999. 옻나무 추출물의 생리활성 이용에 대한 연구 : 옻나무 추출물의 생물학적 기능. 한국식품과학회지 31(1): 238-245.
  9. 전병덕, 이윤경, 이수천, 김판기, 정남철, 김병오, 류승필. 2008. 옻나무 추출액 섭취가 흰쥐의 혈중 지질 및 항산화 능력에 미치는 영향. 한국임학회지 97(4): 478-484.
  10. 정형진, 김은희, 이건주, 정규영, 임종국, 유정민, 심영은, 박재호. 2001. 옻나무류의 항산화력 항산화 효소 활성. 자원식물학회지 14(3): 220-228.
  11. 차재영, 조영수. 2000. 옻나무 수피 추출물이 마우스의 지질농도 및 지질과산화에 미치는 영향. 생명과학회지 10(5): 467-474.
  12. Alessio, H.M., and Goldfarb, A.H. 1988. Lipid peroxidation and scavenger enzymes during exercise: adaptative response to training, J. Appl. Physiol. 64: 1333-1336.
  13. Finaud, J., Lac, G., and Filaire, E. 2006. Oxidative stress: relationship with exercise and training. Sports Med. 36(4): 327-358. https://doi.org/10.2165/00007256-200636040-00004
  14. Goldhammer, E., Tanchilevitch, A., Maor, I., Beniamini, Y., Rosenschein, U., and Sagiv, M. 2005. Exercise training modulates cytokines activity in coronary heart disease patients. Int. J. Cardiol. 100: 93-99. https://doi.org/10.1016/j.ijcard.2004.08.073
  15. Gunduz, F., Senturk, U.K., Kuru, O., Aktekin, B., and Aktekin, M.R. 2004. The effect of one year's swimming exercise on oxidant stress and antioxidant capacity in aged rats. Physiol. Res. 53: 171-176.
  16. Harris, E.D. 1992. Regulation of antioxidant enzymes. FASEB J. 6: 2675-2783.
  17. Havel, P.J. 2002. Control of energy homeostasis and insulin action by adipocyte hormones: leptin, acylation stimulating protein, and adiponectin. Curr. Opin. Lipidol. 13(1): 51-59. https://doi.org/10.1097/00041433-200202000-00008
  18. Hertog, M.G., Feskens, E.J., and Kromhout, D. 1997. Antioxidant flavonols and coronary heart disease risk. Lancet 349(9053): 699.
  19. Hong, D.H., Han, S.B., Lee, C.W., Park, S.H., Jeon, Y.J., Kim, M.J., Kwak, S.S., and Kim, H.M. 2000. Cytotoxicity of urushiols isolated from sap of Korean lacquer tree (Rhus verniciflua Stokes). Arch. Pharma. Res. 22: 638-641.
  20. Jackson, M.J., Edwards, R.H.T., and Synons, M.C.R. 1985. Electron spin resonance studies of intact mammalian skeletal muscle. Biochim. Biophys. Acta. 847: 185-190. https://doi.org/10.1016/0167-4889(85)90019-9
  21. Jae, S.Y., Fernhall, B., Heffernan, K.S., Jeong, M., Chun, E.M., Sung, J., Lee, S.H., Lim, Y.J., and Park, W.H. 2006. Effects of lifestyle modifications on C-reactive protein: contribution of weight loss and improved aerobic capacity. Metabolism 55(6): 825-831. https://doi.org/10.1016/j.metabol.2006.02.010
  22. Joseph, W.S., Brian, D.B. and Marissa, E.O. 2007. Exercise training decreases rat heart mitochondria free radical generation but does not prevent $Ca^{2+}$-induced dysfunction. J. Appl. Physiol. 102(5): 1793-1798. https://doi.org/10.1152/japplphysiol.00849.2006
  23. Jung, C.H., Kim, J.H., Hong, M.H., Seog, H.M., Oh, S.H., Lee, P.J., Kim, G.J., Kim, H.M., Um, J.Y., and Ko, S.G. 2007. Phenolic-rich fraction from Rhus verniciflua Stokes (RVS) suppress inflammatory response via NFkappaB and JNK pathway in lipopolysaccharide-induced RAW 264.7 macrophages. J. Ethnopharmacol. 110(3): 490-497. https://doi.org/10.1016/j.jep.2006.10.013
  24. Kim, I.T., Park, Y.M., Shin, K.M., Hab, J.H., Choi, J.W., Jung, H.J., Park, H.J., and Lee, K.T. 2004. Anti-inflammatory and anti-nociceptive effects of the extract from Kalopanax, Pueraria thunbergiana and Rhus verniciflua. J. Ethnopharmacol. 94(1): 165-173. https://doi.org/10.1016/j.jep.2004.05.015
  25. Kim, J.H. 2007. Utility assessment of whey protein and $\beta$-carotene supplementation for muscle mass, 1RM, immunoglobulin and white blood cell ratio during high intensity muscular resistance training. J. Sport Leisure Studies 34: 1217-1225.
  26. Kohut, M.L., McCann, D.A., Russell, D.W., Konopka, D.N., Cunnick, J.E., Franke, W.D., Castillo, M.C., Reighard, A.E., and Vanderah, E. 2006. Aerobic exercise, but not flexibility/ resistance exercise, reduces serum IL-18, CRP, and IL- 6 independent of beta-blockers, BMI, and psychosocial factors in older adults. Brain Behav. Immun. 20(3): 201-209. https://doi.org/10.1016/j.bbi.2005.12.002
  27. Lanigan, R.S., and Yamarik, T.A. 2002. Final report on the safety assessment of BHT. Int. J. Toxicol. 21(2): 19-94.
  28. Larson, A.I., Aukrust, P., Aarsland, T., and Dickstein, K. 2001. Effects of aerobic exercise training on plasma levels of tumor necrosis factor alpha in patients with heart failure. Am. J. Cardiol. 88(7): 805-808. https://doi.org/10.1016/S0002-9149(01)01859-8
  29. Lee, J.C., and Lim, K.T. 2000. Screening of antioxidant and antimicrobial effects freom Rhus verniciflua Stokes (RVS) ethanolic extract. Food Sci. Biotechnol. 9: 139-145.
  30. Lee, J.D., Huh, J., Jeon, E.G., Yang, H.R., Woo, H.S., Choi, D.Y., and Park, D.S. 2009a. Flavonol-rich RVHxR from Rhus verniciflua Stokes and its major compound fisetin inhibits inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells and in vivo models. Int. J. Immunopharmacol 9(3): 268-276. https://doi.org/10.1016/j.intimp.2008.11.005
  31. Lee, J.H., Lee, H.J., Lee, H.J., Choi, W.C., Yoon, S.W., Ko, S.G., Ahn, K.S., Choi, S.H., Ahn, K.S., Lieske, J.C., and Kim, S.H. 2009b. Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice. Phytomedicine 16(2): 188-197. https://doi.org/10.1016/j.phymed.2008.10.009
  32. Lee, S.H., Seo, G.S., and Sohn, D.H. 2004. Inhibition of lipopolysaccharide -induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem. Biophys. Res. Commun. 323(1): 125-132. https://doi.org/10.1016/j.bbrc.2004.08.063
  33. Lim, K.T., Lee, S.J., Heo, K.S., and Lim. K. 2003. Effects of glycoprotein isolated from Rhus verniciflua stokes on TPA-induced apoptosis and production of cytokines in cultured mouse primary splenocytes. Toxicol. Lett. 145(3): 261-271. https://doi.org/10.1016/S0378-4274(03)00304-7
  34. Marfella, R., Esposito, K., Siniscalchi, M., Cacciapuoti, F., Giugliano, F., Labriola, D., Ciotola, M., Di Palo, C., Misso, L., and Giugliano, D. 2004. Effect of weight loss on cardiac synchronization and proinflammatory cytokines in premenopausal obese women. Diabetes Care 27(1): 47-52. https://doi.org/10.2337/diacare.27.1.47
  35. McMurray, R.G., Ainsworth, B.E., Harrell, J.S., Griggs, T.R., and Williams, O.D. 1998. Is physical activity or aerobic power more influential on reducing cardiovascular disease risk factors? Med. Sci. Sports Exer. 30(10): 1521-1529. https://doi.org/10.1097/00005768-199810000-00009
  36. Monzillo, L.U., Hamdy, O.E., Horton, S., Ledbury, S., Mullooly, C., Jarema, C., Porter, S., Ovalle, K., Moussa, A., and Mantzoros, C.S. 2003. Effect of lifestyle modification on adipokine levels in obese subjects with insulin resistance. Obes. Res. 11(9): 1048-1054. https://doi.org/10.1038/oby.2003.144
  37. Rajala, M.W., and Scherer, P.E. 2003. Minireview: The adipocyte-at the crossroads of energy homeostasis, inflammation, and atherosclerosis. Endocrinol. 144(9): 3765-3773. https://doi.org/10.1210/en.2003-0580
  38. Ryan, A.S., and Nicklas, B.J. 2004. Reductions in plasma cytokine levels with weight loss improve insulin sensitivity in overweight and obese postmenopausal women. Diabetes care 27(7): 1699-1705. https://doi.org/10.2337/diacare.27.7.1699
  39. Sen, C.K. 1995. Oxidants and antioxidants in exercise. J. Appl. Physiol. 79(3): 675-686.
  40. Sowers, J.R. 2003. Obesity as a cariovascular risk factor. Am. J. Med. 115(8): 375-415.
  41. Suzuki, K., Ito. Y., Ochiai, J., Kusuhara, Y., Hashimoto, S., Tokudome, S., Kojima, M., Wakai, K., Toyoshima, H., Tamakoshi, K., Watanabe, Y., Hayakawa, N., Maruta, M., Watanabe, M., Kato, K., Ohta, Y., and Tamakoshi, A. 2003. Relationship between obesity and serum markers of oxidative stress and inflammation in Japanese. Asian Pac. J. Cancer Prev. 4(3): 259-266.
  42. Thomas, M.J. 2000. The role of free radicals and antioxidants. Nutrition 16(7-8): 716-718. https://doi.org/10.1016/S0899-9007(00)00343-9
  43. Trenam, C.W., Blake, D.R. and Morris, C.J. 1992. Skin inflammation: reactive oxygen species and the role of iron. J. Invest. Dermatol. 99(6): 675-682. https://doi.org/10.1111/1523-1747.ep12613740
  44. Urso, M.L., and Clarkson, P.M. 2003. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189(1-2): 41-54. https://doi.org/10.1016/S0300-483X(03)00151-3
  45. Valko, M., Leibfritz, D., Moncol, J.M., Cronin, T., Mazur, M., and Telser, J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39(1): 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  46. Vieira, V.J., Hu, L.R., Valentine, J., McAuley, E., Evans, E.M., Baynard, T., and Woods, J.A. 2009. Reduction in trunk fat predicts cardiovascular exercise training-related reductions in C-reactive protein. Brain Behav. Immun. 23(4): 485-491. https://doi.org/10.1016/j.bbi.2009.01.011
  47. Vincent, H.K., Powers, S.K., Stewant, D.J., Demirel, H.A., Shanely, R.A., and Naito. H. 2000. Short-term exercise training improves diaphragm antioxidant capacity and endurance. Eur. J. Appl. Physiol. 81(1-2): 67-74. https://doi.org/10.1007/PL00013799