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Isolation and Characterization of Feather Keratin-Degrading Bacteria and Plant Growth-Promoting Activity of Feather Hydrolysate

우모 케라틴 분해세균의 분리, 특성 및 우모 분해산물의 식물 생육촉진 효과

  • Jeong, Jin-Ha (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Na-Ri (College of Natural Resources and Life Science, Pusan National University) ;
  • Kim, Jeong-Do (College of Natural Resources and Life Science, Pusan National University) ;
  • Jeon, Young-Dong (College of Natural Resources and Life Science, Pusan National University) ;
  • Park, Ki-Hyun (College of Natural Resources and Life Science, Pusan National University) ;
  • Oh, Dong-Joo (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Chung-Yeol (College of Natural Resources and Life Science, Pusan National University) ;
  • Son, Hong-Joo (College of Natural Resources and Life Science, Pusan National University)
  • 정진하 (부산대학교 생명자원과학대학) ;
  • 이나리 (부산대학교 생명자원과학대학) ;
  • 김정도 (부산대학교 생명자원과학대학) ;
  • 전영동 (부산대학교 생명자원과학대학) ;
  • 박기현 (부산대학교 생명자원과학대학) ;
  • 오동주 (부산대학교 생명자원과학대학) ;
  • 이충열 (부산대학교 생명자원과학대학) ;
  • 손홍주 (부산대학교 생명자원과학대학)
  • Received : 2010.09.07
  • Accepted : 2010.10.11
  • Published : 2010.10.31

Abstract

This study was conducted to isolate and characterize a novel feather-degrading bacterium producing keratinase activity. A strain K9 was isolated from soil at poultry farm and identified as Xanthomonas sp. K9 by phenotypic characters and 16S rRNA gene analysis. The cultural conditions for the keratinase production were 0.3% fructose, 0.1% gelatin, 0.04% $K_2HPO_4$, 0.06% $KH_2PO_4$, 0.05% NaCl and 0.01% $FeSO_4$ with an initial pH 8.0 at $30^{\circ}C$ and 200 rpm. In an optimized medium containing 0.1% chicken feather, production yield of keratinase was approximately 8-fold higher than the yield in basal medium. The strain K9 effectively degraded chicken feather meal (67%) and duck feather (54%), whereas human nail and human hair showed relatively low degradation rates (13-22%). Total free amino acid concentration in the cell-free supernatant was about 25.799 mg/l. Feather hydrolysate produced by the strain K9 stimulated growth of red pepper, indicating Xanthomonas sp. K9 could be not only used to increase the nutritional value of chicken feather but also a potential candidate for the development of natural fertilizer applicable to crop plant soil.

Keywords

Feather;Fertilizer;Keratin;Keratinase

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