Optimal Culture Conditions for Photosynthetic Microalgae Nannochloropsis oculata

광합성 미세조류 Nannochloropsis oculata의 최적배양 조건

  • Park, Hyun-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jin, Eun-Jung (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jung, Tae-Man (Marine-Bio Center, Silla University) ;
  • Joo, Hyun (Department of Physiology and Integrated Biosystems, College of Medicine, Inje University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 박현진 (신라대학교 공과대학 생명공학과) ;
  • 진은정 (신라대학교 공과대학 생명공학과) ;
  • 정태만 (에이엠 바이오(주)) ;
  • 주현 (인제대학교 생리학교실) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Received : 2010.09.06
  • Accepted : 2010.09.28
  • Published : 2010.12.10

Abstract

Microalgae has been seen all over the seawater and several species are used for human food. Specially, Nannochloropsis oculata, a photosynthetic microalgae, has been focused for a vast array of valuable nutritious compounds. In order to find high mass Nannochloropsis oculata culture conditions, some of important growth factors of pH, temperature, culture media, and $CO_2$ effect were tested. The optimal growth condition was found to be as follows : 3% artificial seawater, initial pH 8.5, and temperature $25^{\circ}C$. The alga mass and chlorophyll content were dramatically increased by applying 5% flue $CO_2$ gas (1.50 g/L algae in a continuous $CO_2$ flue; 0.76 g/L alga without $CO_2$). It was shown that the chlorophyll biosynthesis was also closely associated with alga growth.

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