Role of HOXA Gene in Human Endometrial Decidualization

인간 자궁내막의 탈락막화에서 HOXA10 유전자의 역할

  • Lee, Chang-Se (Laboratory of Molecular Oncology, Cheil General Hospital, Kwandong University College of Medicine) ;
  • Park, Dong-Wook (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Kwandong University College of Medicine) ;
  • Park, Chan-Woo (Department of Obstetrics and Gynecology, Cheil General Hospital, Kwandong University College of Medicine) ;
  • Kim, Tae-Jin (Department of Obstetrics and Gynecology, Cheil General Hospital, Kwandong University College of Medicine)
  • 이창세 (관동대학교 의과대학 제일병원 분자종양학 연구실) ;
  • 박동욱 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ;
  • 박찬우 (관동대학교 의과대학 제일병원 산부인과) ;
  • 김태진 (관동대학교 의과대학 제일병원 산부인과)
  • Received : 2010.06.07
  • Accepted : 2010.08.03
  • Published : 2010.09.30

Abstract

Objective: This study was performed to clarify the role of HomeoboxA (HOXA) and its related signaling molecules in the decidualization of primary cultured endometrial cells. Methods: Human endometrial tissues were obtained by curettage of hysterectomy specimens from patients with conditions other than endometrial diseases. Tissues were minced and digested with Trypsin-EDTA for 20 min, $37^{\circ}C$. Cells were cultured with DMEM/F12 medium in $37^{\circ}C$, 5% $CO_2$ incubator for 24 hrs. Cells were treated with HOXA10 siRNA and added transforming growth factor (TGF)-${\beta}1$ (10 ng/mL) for 48 hrs to induces decidualization in vitro. Reverse transcription polymerase chain reaction analysis was accomplished to observe the expression of HOXA10, prolactin, cyclooxygenase (COX)-2, peroxisome proliferatoractivated receptor (PPAR)-$\gamma$, and wingless-type MMTV integration site family (Wnt). Results: HOXA10 expression was increased (1.8 fold vs. non-treated control) in TGF-${\beta}1$ treated cells. Decidualization marker, prolactin, was significantly increased in TGF-${\beta}1$ treated cells compared with HOXA10 siRNA treated cells. Endometrial cell differentiation marker, COX-2 was down-regulated by HOXA10 siRNA even if cells were treated with TGF-${\beta}1$. Wnt4 was down-regulated by treated with HOXA10 siRNA, this expression patters was not changed by TGF-${\beta}1$. Expression of PPAR-$\gamma$ was down regulated by TGF-${\beta}1$ in regardless of HOXA10 siRNA treatment. Conclusion: TGF-${\beta}1$ which is induced by progesterone in endometrial epithelial cells may induces stromal cell decidualization via HOXA10 and Wnt signaling cascade.

Acknowledgement

Supported by : 한국연구재단

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