Biological Control of Garlic Blue Mold using Pantoea agglomerans S59-4

Pantoea agglomerans S59-4를 이용한 마늘 푸른곰팡이병의 생물학적 방제

  • Kim, Yong-Ki (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Hong, Sung-Jun (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Jee, Hyung-Jin (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Jong-Ho (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Han, Eun-Jung (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Kyung-Seok (Agricultural Microbiology Div., Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Sang-Yeob (Agricultural Microbiology Div., Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Seong-Don (Research Coordination Division, Research Policy Bureau, RDA)
  • 김용기 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 홍성준 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 지형진 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박종호 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 한은정 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박경석 (농업생물부 농업미생물과) ;
  • 이상엽 (농업생물부 농업미생물과) ;
  • 이승돈 (농촌진흥청 연구정책국 연구조정과)
  • Received : 2010.04.23
  • Accepted : 2010.05.13
  • Published : 2010.06.30

Abstract

S59-4 isolate was evaluated as a potential biocontrol agent using in vivo wounded garlic bulb assay. When the spore suspension ($10^5$ spores/$m\ell$) of Penicillium hirsutum was co-inoculated with cell suspension of S59-4 isolate on wounded garlics, the isolate showed high suppressive effect to disease development. The isolate was identified as Pantoea agglomerans S59-4(Pa59-4) through Biolog system. Furthermore, soaking garlic bulbs in the suspension of Pa59-4 significantly reduced garlic decay caused by P. hirsutum. The optimal concentration of Pa59-4 for controlling garlic blue mold was $10^7\sim10^8$ cfu/$m\ell$. And suppressive effect of Pa59-4 on garlic storage decay reduced as inoculation concentration of Penicillium hirsutum increased. In addition in order to investigate population dynamics of Pa59-4 on application site of garlic cloves, two antibiotic markers, pimaricin and vancomycin were selected. Bacterial density of Pa59-4 on the wounded garlic cloves increased continuously both under room temperature condition and low temperature condition until 30days after application of Pa59-4, meanwhile that of Pa59-4 on intact garlic cloves increased until 15days after application of Pa59-4 and thereafter decreased continuously. Two culture media for mass-production of Pa59-4, LB medium and TSB medium, were selected. By-product of bio-fungicide formulated by mixing white carbon and bacterial suspension of Pa59-4 suppressed by 40 to 50% garlic blue mold. Above results suggest that Pa59-4 be a promising control agent against garlic blue mold.

Keywords

Pantoea agglomerans S59-4;garlic blue mold;Biological control;mass production

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