Control Effects of Micromonospora sp. AW050027 by Media Optimization and Microbial Treatment Against Pine Wood Nematode

Micromonospora sp. AW050027 균주의 배지최적화 및 미생물제제 처리에 의한 소나무재선충 방제효과

  • Park, Dong-Jin (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chang, Yong-Ha (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Jin (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • 박동진 (한국생명공학연구원 미생물자원센터) ;
  • 이재찬 (한국생명공학연구원 미생물자원센터) ;
  • 장용하 (한국생명공학연구원 미생물자원센터) ;
  • 김창진 (한국생명공학연구원 미생물자원센터)
  • Received : 2010.04.23
  • Accepted : 2010.05.11
  • Published : 2010.06.30

Abstract

Nematicidal activity against pine wood nematode, Bursaphelenchus xylophilus, was tested in the pot and field by the treatment of microbial formulation after media optimization. The optimized media composition was glycerol 10 g, soybean meal 10 g, NaCl 1 g, $CaCO_3$ 2 g, $K_2HPO_4$ 0.125 g per liter and microbial complex formulation was made with liquid and powder type. Most effective antibiotics against symbiotic microorganism with nematode, kanamycin, was added to the formulation. The control effects against pine wood nematodes were checked by pot test and field test. In the result of treatment by trunk injection, five times treatment was more effective than one time and the treatment with the formulation of concentrated culture supernatant was the most effective in the nematicidal activity showing below 10% mortality in pine tree.

Keywords

Micromonospora sp.;Bursaphelenchus xylophilus;nematode;formulation;optimization

Acknowledgement

Grant : 산림과학 기술개발사업

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