Determination of Fomesafen Residue in Agricultural Commodities Using HPLC-UVD/MS

HPLC-UVD/MS를 이용한 농산물 중 fomesafen의 분석

  • Lee, Su-Jin (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Hwang, Young-Sun (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Young-Hak (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Nam, Mi-Young (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Hong, Seung-Beom (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Yun, Won-Kap (Gyeongbuk Institute for Marine Bioindustry) ;
  • Kwon, Chan-Hyeok (National Institute of Food and Drug Evaluation) ;
  • Do, Jung-A (National Institute of Food and Drug Evaluation) ;
  • Im, Moo-Hyeog (Korea Food and Drug Administration) ;
  • Lee, Young-Deuk (Division of Life and Evnironmental Science, Daegu University) ;
  • Choung, Myoung-Gun (Dept. of Herbal Medicine Resource, Kangwon National University)
  • 이수진 (강원대학교 생약자원개발학과) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 김영학 (강원대학교 생약자원개발학과) ;
  • 남미영 (강원대학교 생약자원개발학과) ;
  • 홍승범 (강원대학교 생약자원개발학과) ;
  • 윤원갑 (경북해양바이오산업연구원) ;
  • 권찬혁 (식품의약품안전평가원 화학물질과) ;
  • 도정아 (식품의약품안전평가원 화학물질과) ;
  • 임무혁 (식품의약품안전청 식품기준과) ;
  • 이영득 (대구대학교 생명환경학부) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • Received : 2010.05.03
  • Accepted : 2010.05.17
  • Published : 2010.06.30

Abstract

Fomesafen is a selective herbicide, and used to control annual and perennial broad-leaf grass on soybean and fruit fields in USA and China, but not introduced in Korea yet. So, MRL (Maximum Residue Level), and analytical method of fomesafen were not establishment in Korea. Therefore, this experiment was conducted to establish a determination method for fomesafen residue in crops using HPLC-UVD/MS. Fomesafen residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover fomesafen from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The fomesafen was quantitated by HPLC with UVD, using a Shiseido CAPCELL-PAK UG C18 column. The crops were fortified with fomesafen at 3 levels per crop. Mean recovery ratio were ranged from 87.5% for a 0.4 ppm in hulled rice to 102.5% for a 0.4 ppm in apple. The coefficients of variation were ranged from 0.6% for a 2.0 ppm in hulled rice to 7.7% for a 0.04 ppm in green pepper. Quantitative limit of fomesafen was 0.04 mg/kg in representative 5 crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fomesafen in agricultural commodities.

Keywords

fomesafen;HPLC-UVD/MS;residue;agricultural commodities

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