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Antifungal Effect of Brachyglottis repanda Ethanol Extract

  • Yook, Chan-Nam (Department of Food Science, Wonkwang Health Science) ;
  • Na, Young-Soon (Division of Bionanochemistry, College of Natural Sciences, Wonkwang University) ;
  • Choi, Hwa-Jung (Department of Clinical Pathology, Daejeon Health Science College) ;
  • You, Il-Soo (Department of Nanopolymer, National Chonbuk University) ;
  • Baek, Jong-Min (R&D Center, BRNScience Co. LTD., Seoul National University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Received : 2010.01.03
  • Accepted : 2010.03.24
  • Published : 2010.06.01

Abstract

The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at $150{\mu}g$/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney). However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 ($IC_{50}$, > 62,500 ng/ml). Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% $CH_3CN/H_2O$, 100% $CH_3CN$, and 50% $CH_3CN/H_2O$(SM 2 at $150{\mu}g$/disk). The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, $IC_{50}$ 10,051 ng/ml at $75{\mu}g$/disk). These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.

References

  1. Allan, H.H. (1960). Flora of New Zealand. Indigenoue, Rracheopphyta, Psilopsida, Lycopsida, Filicopsida, Gymnopermae, Dicotyledones; DSIR: Wellington, Vol. 1.
  2. Baek, S.H., Shin, J.H., Chung, W.Y. and Han, D.S. (2000). Antitumor evaluation of cannabidiol and its derivatives by colorimetric methods. J. Toxicol. Pub. Health, 16, 101-107.
  3. Baek, S.H., Lim, JA., Kwag, J.S., Lee, H.O., Chun, H.J., Lee, J.H. and Perry, N.B. (2003). Screening for biological activity of crude extract and fractions from Brachyglottis monroi. Kor. J. Orien. Physiol. Pathol., 17, 826-828.
  4. Bloor, S.J. and Gainsford, G.J. (1993). A novel clerodane-ascorbate adduct from Brachyglottis bidwillii. Aust. J. Chem., 46, 1099-1104. https://doi.org/10.1071/CH9931099
  5. Becconsall, J.K. (1993). Basic One- and Two-Dimensional NMR Spectroscopy, 2nd Ed., VCH Publishers, New York, pp. 41-57.
  6. Connor, H.E. and Edger, E. (1987). Name changes in the indigenous New Zealand flora. 1960 - 1986 and Nomina Nova IV, 1983 - 1986. N. Z. J. Bot., 25, 2255-2258.
  7. Choi, H.J., Song, J.H., Kwon, D.H. and Baek, S.H. (2010). Antiviral and anticancer activities of 13(E)-Labd-13-ene-8a,15-diol isolated from Brachyglottis monroi. Phytother Res., 24, 169-174.
  8. Keepers, Y.P., Pozao, P.E., Peters, G.T., Otte, J.A., Winograd, B. and Pinedo, H.M. (1991). Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. Eur. J. Cancer, 27, 897-900. https://doi.org/10.1016/0277-5379(91)90142-Z
  9. Kwag, J.S., Na, Y.S., Perry, N.B., Kim, H.M. and Baek, S. H. (2004). Pharmacological effects of bioactive fractions from Brachyglottis monroi. Kor. J. Orien. Physiol. Pathol., 18, 260-264.
  10. Kim, M.J., Lee, J.S. and Baek, S.H. (2009). In vitro biological activity of germacranolide sesquiterpene lactones. Orien. Pharm. Exp. Med., 9, 192-199. https://doi.org/10.3742/OPEM.2009.9.2.192
  11. Lim, J.A, Kwag, J.S., Yu, B.S. and Baek, B.S. (2004). Cytotoxic activity of 13(E)-labd -13-ene-8a,15-diol. Kor J. Orien. Physiol. Pathol, 18, 1163-1166.
  12. Mosmann, T. (1983). Rapid colorimetric assays for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  13. Mortimer, P.H. and White, E.P. (1967). Hepatotoxic substance in Brachyglottis repanda. Nature, 214, 1255-1256. https://doi.org/10.1038/2141255a0
  14. Riley, M. (1994). Maori Healing and Herbal. Paraaraumu, New Zealand: Viking Sevenseas N. Z. Ltd. pp 481.
  15. Schroeder, A.C., Hughes, R.G. Jr. and Bloch, A. (1981). Synthesis and biological effects of acyclic pyrimicine nucleoside analogues. J. Med. Chem., 24, 1078-1083. https://doi.org/10.1021/jm00141a012
  16. Shin, J.H., Oh, J.H., Park, H.J., Kim, N.K., Jeong, Y.H., Lee, S.I. and Baek, S.H. (2001). Isolation of cytotoxic component from Trichocolea hatcheri. Orien. Pharm. Exp. Med., 1, 49-56.
  17. Yook, C.N., Lee, J.S. and Baek, S.H. (2007). Pharmacological screening of crude extracts from medicinal plants (II). Kor. J. Orien. Physiol. Pathol., 21, 1004-1009.
  18. Williams, D.H. and Fleming, J. (1995). Spectroscopic Methods in Organic Chemistry, New York: Mcgraw-Hill; pp. 28-62.

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