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Antiulcer activity of Trichosanthes cucumerina linn. against experimental gastro-duodenal ulcers in rats

  • Galani, VJ (Department of Pharmacology, A. R. College of Pharmacy) ;
  • Goswami, SS (Department of Pharmacology, L. M. College of Pharmacy) ;
  • Shah, MB (Department of Pharmacognosy, L. M. College of Pharmacy)
  • Received : 2010.02.08
  • Accepted : 2010.05.26
  • Published : 2010.09.30

Abstract

Trichosanthes cucumerina Linn. (cucurbitaceae) is widely used in Indian folk medicine for variety of disease conditions. The aim of present study was to evaluate the antiulcer activity of 50% ethanolic extract of fruits of Trichosanthes cucumerina Linn. (TCFE) using various experimental models of gastric and duodenal ulceration in rats. Oral administration of 50% ethanolic extract of fruits of Trichosanthes cucumerina Linn. was evaluated in rats against ethanol, aspirin and pylorus ligated gastric ulcers as well as cysteamine-induced duodenal ulcers. In all the models studied, the antiulcer activity of TCFE compared with that of cimetidine (100 mg/kg, p.o.), an $H_2$ receptor antagonist. TCFE showed significant antiulcer activity in ethanol-induced and aspirin-induced gastric ulcer models. In 19 h pylorus ligated rats, significant reduction in ulcer index, total acidity and pepsin activity was observed with TCFE, when compared with the control group. Mucosal defensive factors such as pH, mucin activity and gastric wall mucous content was found to be increased with TCFE. TCFE was also, afforded remarkable protection in cysteamine-induced duodenal lesions. The antiulcer activity of TCFE was comparable with that of cimetidine. Thus, TCFE possess significant antiulcer activity against both gastric and duodenal ulcers in rats. The antiulcer activity may be attributed to its cytoprotective action and inhibition of acid secretary parameters.

Keywords

References

  1. Adebooye OC. (2008) Phyto-constituents and antioxidant activity of the pulp of snake tomato Trichosanthes cucumerina L. Afr. J. Trad. CAM. 5, 173-179.
  2. Alkofahi A, Atta AH. (1999) Pharmacological screening of the antiulcerogenic effects of someJordanian Medicinal Plants in rats. J. Ethnopharmacol. 65, 341-345.
  3. Ariyphisi I, Toshiharu A, Sugimura F, Abe M, Matsuo Y, HondaT. (1986) Recurrence during maintenance therapy with histamine $H_2$receptors antagonist in cases of gastric ulcers. Nikon.Univ. J. Med. 28, 69-74.
  4. Borelli F, Izzo AA. (2000) The plant kingdom as a source of anti-ulcer remedies. Phytother. Res. 14, 581-591. https://doi.org/10.1002/1099-1573(200012)14:8<581::AID-PTR776>3.0.CO;2-S
  5. Briden S, Flemstrom G, Kivilaasko E. (1985) Cysteamine and propionitrile inhibit the rise of duodenal mucosal alkaline secretion in response to luminal acid in rats. Gastroenterology. 24, 104-107.
  6. Corne SJ, Motrisser SM. (1974) A method for the quantitative estimation of gastric barrier mucus. J. Physiol. 242, 116-117.
  7. Debnath PK, Gode KD, Govinda DAS, Sanyal AK. (1974) Effect of propranolol on gastric secretion in albino rats. Br. J. Pharmacol. 51, 213-216. https://doi.org/10.1111/j.1476-5381.1974.tb09649.x
  8. Dharmani P, Palit G. (2006) Exploring Indian medicinal plants for antiulcer activity. Ind. J. Pharmacol. 38, 95-99. https://doi.org/10.4103/0253-7613.24613
  9. Edeoga HO, Osuagwu GGE, Omosun G, Mbaebie BO, Osuagwu AN. (2010) Pharmaceutical and therapeutic potential of some wild cucurbitaceae species from South-east Nigeria. Rec. Res. Sci. Tech. 2, 63-68.
  10. Fridovich I. (1978) The biology of free radicals. Science. 201, 875-880. https://doi.org/10.1126/science.210504
  11. Ganguly AK, Bhatnagar OP. (1973) Effect of bilateral adrenalectomy on the production of restraint ulcer in the stomach of albino rats. Canad. J. Physiol. Pharmacol. 51, 748-750. https://doi.org/10.1139/y73-113
  12. Glass GBJ, Slomiany BL, Slomiany A. (1979) Biochemical and pathological derangements of the gastrointestinal tract following acute and chronic digestion of ethanol. In: Biochemistry and pharmacology of ethanol, edited by Majchrowicz E, Noble, EP, p.551-586, Plenum Press, New York.
  13. Goel RK, Chakrabarti A, Sanyal AK. (1985) The effect of biological variables on the antiulcerogenic effect of vegetable plantain banana. Planta Medica. 2, 85-88.
  14. Goel RK, Bhattacharya SK. (1991) Gastroduodenal mucosal defence and mucosal protective agents. Ind. J. Exp. Biol. 29, 701-714.
  15. Gurbuz I, Akyuz C, Yesilada E, Sener B. (2000) Antiulcerogenic effect of Momordica charant.ia L. fruits on various ulcer models in rats. J. Ethnopharmacol. 71, 77-82. https://doi.org/10.1016/S0378-8741(99)00178-6
  16. Harit M, Rathee PS. (1996) Antifungal activity of the unsaponifiable fractions of the fixed oils of (Trichosanthes) seeds. Asian. J. Chem. 8, 180-182.
  17. Hariti M, Rathee PS. (1995) Antibacterial activity of the unsaponifiable fractions of the fixed oils of (Trichosanthes) seeds. Asian. J. Chem. 7, 909-911.
  18. Hawk PB, Oser BC, Summerson WH. (1954) Practical Physiological chemistry, 13th edn, pp.466-487, Blakiston Company Inc., Toronto, New York.
  19. Hemmati H, Rezvani A, Djahanjuiri B. (1973) Prevention of aspirin induced ulceration in rats with ${\alpha}$-methyldopa and disulfiram. Pharmacology. 9, 374-378. https://doi.org/10.1159/000136411
  20. Hiraishi H, Shimuda T, Irey KH, Terano A. (1999) Role of antioxidant defenses against ethanol-induced damage in cultured rat gastric epithelial cells. J. Pharmacol. Exp. Ther. 289, 103-109.
  21. Irwin S. (1962) Drug screening and evaluative procedures. Science. 136, 123-136. https://doi.org/10.1126/science.136.3511.123
  22. Kage DN, Malashetty VB, Seetharam YN, Suresh P, Patil SB. (2009) Effect of ethanol extract of whole plant of Trichosanthes cucumerina var. cucumerina L. on gonadotropins, ovarian follicular kinetics and estrous cycle for screening of antifertility activity in albino rats. Int. J. Morphol. 27, 173-182.
  23. Kirtikar KR, Basu BD. (1935) Indian Medicinal plants, Vol 2, pp. 1112, Lalit Mohan Basu, Allahabad, India.
  24. Kirana H, Srinivasan B. (2008) Trichosanthes cucurmerina Linn. improves glucose tolerance and tissue glycogen in non insulin dependent diabetes mellitus induced rats. Indian. J. Pharmacol. 40, 103-108.
  25. Kitagawa H, Kurahashi K, Fujiwara M, Kohei H. (1978) Antiulcerogenic effect of a pyrido-benzodazepine derivative (L-S519) on experimental ulcers. Arzneim. Forsch. 28, 2122-2127.
  26. Kolte RM, Bisan VV, Jangde CR, Bhalerao AA. (1996) Anti-inflammatory activity of root tubers of trichosanthes cucumerina (LINN) in mouse's hind paw oedema induced by carrageenan. Indian. J. Indigeneous. Med. 18, 117-121.
  27. Kulkarni SK, Goel RK. (1996) Gastric antiulcer activity of UL-409 in rats. Indian J. Exp. Biol. 34, 683-686.
  28. Lowry OH, Rosenberg NJ, Farr AL, Randall RJ. (1951) Protein measurement with folin reagent. J. Biol. Chem. 193, 265-275.
  29. McGrath MS, Luk KC, Abrams HD, Gaston I, Santulli S, Caldwell SE, Piatak M, Lifson JD. (1992) Antiviral studies with trichosanthin, a plant derived single chain ribosome inactivating protein. In: Natural Products as Antiviral Agents, edited by Chu CK, Cutler H. p. 171-193, Plenum Press, New York.
  30. Mozsik GY, Kiss B, Javor J, Kraus M, Toth E. (1969) Effect of cholinesterase inhibitor treatment on phosphorus and nucleic acid metabolism in the stomach wall. Pharmacology. 2, 45-59. https://doi.org/10.1159/000135998
  31. Nair BR. Investigation on the vanom of South Indian Scorpion Heterometrus Scaber (Ph.D Thesis). Trivendrum (Kerala), University of Kerala, 1974.
  32. Oates PJ, Hakkineu JP. (1988) Studies on the mechanism of ethanol-induced gastric damage in rats. Gastroenterol. 94, 10-21.
  33. Ogawa A, Yabana T. (1993) Pathogenic role of endothelin-1 on ethanol-induced gastric mucosal lesion of rat. Sapporo Igaku Zasshi. 62, 203-211.
  34. Peskar BM, Lange K, Hoppe U, Peskhar BA. (1986) Ethanol-stimulates formation of leukotriene $C_4$ in rat gastric mucosa. Prostaglandins. 31, 283-293. https://doi.org/10.1016/0090-6980(86)90054-7
  35. Peskar BM, Maricic N. (1998) Role of prostaglandins in gastroprotection. Dig. Dis. Sci. 43, S23-S29.
  36. Raskin JB. (1999). Gastrointestinal effects of NSAID therapy. Am. J. Med. 106, 3-12. https://doi.org/10.1016/S0002-9343(98)00338-6
  37. Robert A. (1979) Cytoprotection by prostaglandins. Gastroenterology. 77, 761-767.
  38. Robert A, Nezamis JS, Lancaster C, Hanchar AJ. (1979) Cytoprotection by prostaglandin in rats prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic, NaCl and thermal injury. Gastroenterology. 77, 433-443.
  39. Kumar SS, Kumar RB, G. Krishna Mohan. (2009) Hepatoprotective effect of Trichosanthes cucumerina Var cucumerina L. on carbon tetrachloride induced liver damage in rats. J. Ethnopharmacol. 123, 347-350. https://doi.org/10.1016/j.jep.2009.02.023
  40. Sanyal AK, Mitra PK, Goel RK. (1983) A modified method to estimate dissolved mucosubstances in gasric juice. Ind. J. Exp. Biol. 21, 78-80.
  41. Shay H, Komarov SA, Fcis SE, Meraze D, Gruenstein M, Siplet H. (1973) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology. 5, 43-61.
  42. Szabo S. (1978) Animal model of human disease. Cysteamine induced acute and chronic duodenal ulcers in rats. Am. J. Pathol. 93, 273-276.
  43. Szabo S, Trier JS, Frankel PW. (1981) Sulphydryl compounds may mediate gastric cytoprotection. Science. 214, 200-202. https://doi.org/10.1126/science.7280691
  44. Szelehji I, Brune K. (1988) Possible role of oxygen free radicals in ethanol induced gastric mucosal damage in rats. Dig. Dis. Sci. 33, 865-871. https://doi.org/10.1007/BF01550977
  45. Takeuchi K, Nishikawa H, Okabe S. (1987) Role of local motility changes in the pathogenesis of duodenal ulcers induced by cysteamine in rats. Dig. Dis. Sci. 32, 295-304. https://doi.org/10.1007/BF01297057
  46. Tamblyn R, Berkson L, Dauphinee WD, Gayton D, Grad R, Huang A et al. (1997) Unnecessary prescribing of NSAIDs and the management of NSAID-related gastropathy in medical practice. Ann. Intern. Med. 127, 429-438. https://doi.org/10.1001/archinte.127.3.429
  47. Tanaka H, Takeuchi K, Okabe S. (1989) Effects of the duodenal ulcerogens, mepirizole and cysteamine on gastric motility and emptying in rats. Scand J. Gastroenterol. 24, 104-107.
  48. Tepperman BL, Soper BD. (1990) Effect of sialoadenoectomy onethanol-induced gastric mucosal damage in rat: role of neutrophils. Canad. J. Physiol. Pharmacol. 68, 207-210. https://doi.org/10.1139/y90-032
  49. Tindall HD. (1983) Vegetables in the Tropics, pp. 5-7, AVI Publishing Co., Westport, Connecticut, USA.
  50. Toma W, Hiruma-Lima CA, Guerrer RO, Souza AR. (2005) Preliminary studies of Mammea Americana L (Guttiferae) bark/latex extract point to an effective antiulcer effect on gastric ulcer models in mice. Phytomedicine. 12, 345-350. https://doi.org/10.1016/j.phymed.2003.06.009
  51. Yang X, Zhao Y, Lv Y. (2007) Chemical composition and antioxidant activity of an acidic polysaccharide extracted from Cucurbita moschata Duchesne ex Poiret. J. Agric. Food. Chem. 55, 4684-4690. https://doi.org/10.1021/jf070241r

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