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분자생물학적 분석을 통한 Bt 배추의 토양미생물상 영향 비교평가

Molecular Analysis of Microbial Community in Soils Cultivating Bt Chinese Cabbage

  • 손수인 (농촌진흥청 국립농업과학원) ;
  • 오영주 (농촌진흥청 국립농업과학원) ;
  • 오성덕 (농촌진흥청 국립농업과학원) ;
  • 김민경 (농촌진흥청 국립농업과학원) ;
  • 류태훈 (농촌진흥청 국립농업과학원) ;
  • 이기종 (농촌진흥청 국립농업과학원) ;
  • 서석철 (농촌진흥청 국립농업과학원) ;
  • 백형진 (농촌진흥청 국립농업과학원) ;
  • 박종석 (농촌진흥청 국립농업과학원)
  • 투고 : 2010.09.14
  • 심사 : 2010.09.24
  • 발행 : 2010.09.30

초록

본 연구의 목적은 Bt 배추의 토양미생물 군집에 미치는 영향을 조사하기 위한 것이다. 토양미생물 조사에 앞서 토양 화학성분을 분석한 결과, Bt 배추와 일반품종 배추 근권토양간 화학성분의 유의성 있는 차이는 없는 것으로 조사되었다. 생육 최대 성숙기의 토양미생물 군집밀도를 조사했을 때 Bt 배추 근권토양의 미생물 군집밀도 범위가 일반 배추 근권토양의 미생물 군집밀도 범위내에 속하는 것으로 나타났다. 월별 DGGE 분석결과 일반품종 배추 근권토양 미생물 군집에 비해 Bt 배추 근권토양 미생물 군집의 변이는 없었다. 형질전환 벡터 유래 유전자부위를 이용하여 유전자의 수평이동성을 조사했을 때 Bt 근권토양 유래 DNA에서는 벡터 유래 유전자가 검출되지 않았다.

키워드

Bt Chinese cabbage;Soil microbial community;16S rDNA

과제정보

연구 과제 주관 기관 : 농촌진흥청 국립농업과학원

참고문헌

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피인용 문헌

  1. Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice vol.45, pp.5, 2012, https://doi.org/10.7745/KJSSF.2012.45.5.829
  2. Effects of Protox Herbicide Tolerance Rice Cultivation on Microbial Community in Paddy Soil vol.32, pp.2, 2013, https://doi.org/10.5338/KJEA.2013.32.2.95
  3. Effects of Long-Term Fertilization on Microbial Diversity in Upland Soils Estimated by Biolog Ecoplate and DGGE vol.47, pp.6, 2014, https://doi.org/10.7745/KJSSF.2014.47.6.451
  4. Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum vol.32, pp.4, 2014, https://doi.org/10.7235/hort.2014.13149
  5. Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety vol.54, pp.4, 2011, https://doi.org/10.3839/jabc.2011.048
  6. Effects of Transgenic Soybean Cultivation on Soil Microbial Community in the Rhizosphere vol.30, pp.4, 2011, https://doi.org/10.5338/KJEA.2011.30.4.466
  7. Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Carotenoid-Biofortified Rice Variety vol.31, pp.3, 2012, https://doi.org/10.5338/KJEA.2012.31.3.286