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Molecular Analysis of Microbial Community in Soils Cultivating Bt Chinese Cabbage

분자생물학적 분석을 통한 Bt 배추의 토양미생물상 영향 비교평가

  • 손수인 (농촌진흥청 국립농업과학원) ;
  • 오영주 (농촌진흥청 국립농업과학원) ;
  • 오성덕 (농촌진흥청 국립농업과학원) ;
  • 김민경 (농촌진흥청 국립농업과학원) ;
  • 류태훈 (농촌진흥청 국립농업과학원) ;
  • 이기종 (농촌진흥청 국립농업과학원) ;
  • 서석철 (농촌진흥청 국립농업과학원) ;
  • 백형진 (농촌진흥청 국립농업과학원) ;
  • 박종석 (농촌진흥청 국립농업과학원)
  • Received : 2010.09.14
  • Accepted : 2010.09.24
  • Published : 2010.09.30

Abstract

The aim of this study was to investigate the possible impact of Bt Chinese cabbage on the soil microbial community. Microbial communities were isolated from the rhizosphere of one Bt Chinese cabbage variety and four varieties of conventional ones and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional Chinese cabbages were observed to have an insignificant difference. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures were very similar to each other and this genetic stability of microbial communities was maintained throughout the culture periods. Analysis of dominant isolates in the rhizosphere of transgenic and conventional Chinese cabbages showed that the dominant isolates from the soil of transgenic Chinese cabbage belonged to the Bacilli and Alphaproteobacteria, while the dominant isolates from the soil of conventional cabbage belonged to the Holophagae and Planctomycetacia, respectively. These results indicate that the Bt transgenic cabbage has no significant impact on the soil microbial communities.

Keywords

Bt Chinese cabbage;Soil microbial community;16S rDNA

Acknowledgement

Supported by : 농촌진흥청 국립농업과학원

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