DOI QR코드

DOI QR Code

Effect of Chitosan Solution on the Inhibition of Pseudomonas fluorescens Causing Bacterial Head Rot of Broccoli

  • Li, Bin (State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology) ;
  • Liu, Baoping (State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology) ;
  • Su, Ting (State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology) ;
  • Fang, Yuan (State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology) ;
  • Xie, Guanlin (State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology) ;
  • Wang, Guofen (Institute of Environmental and Plant Protection, Chinese Academy of Tropical Agricultural Sciences) ;
  • Wang, Yanli (Zhejiang Academy of Agricultural Sciences) ;
  • Sun, Guochang (Zhejiang Academy of Agricultural Sciences)
  • Received : 2009.12.25
  • Accepted : 2010.04.04
  • Published : 2010.06.30

Abstract

The in vitro antibacterial properties of two kinds of chitosan solutions and their effect in protection of broccoli from bacterial head rot disease were evaluated. Results showed that the two kinds of chitosan solution at different concentrations exhibited strong antibacterial activity against Pseudomonas fluorescens. However, the antibacterial activity of chitosan A solution increased with the increase of chitosan concentration up to 0.10 mg/ml while the antibacterial activity of chitosan B solution increased with the increase of chitosan concentration up to 0.05 mg/ml. In addition, the antibacterial activity of chitosan A and chitosan B solution of 0.10 mg/ml increased with the incubation time within 12 h and 24 h, respectively. The disease incidence and the lesion diameter of broccoli inoculated with P. fluorescens were significantly reduced when plants were either pretreated or post-treated with six different combinations of chitosan solutions. Overall, the results indicated that the two kinds of chitosan solutions had a potential in controlling bacterial head rot of broccoli.

Keywords

antibacterial activity;bacterial head rot;broccoli;chitosan;Pseudomonas fluoreseens

References

  1. Atia, M. M. M., Buchenauer, H., Aly, A. Z. and Abou-Zaid, M. I. 2005. Antifungal activity of chitosan against Phytophthora infestans and activation of defence mechanisms in tomato to late blight. Biol. Agric. Hortic. 23:175-197. https://doi.org/10.1080/01448765.2005.9755319
  2. Bell, A. A., Hubbard, J. C. and Li, L. 1998. Effects of chitin and chitosan on the incidence and severity of Fusarium yellows of celery. Plant Dis. 82:322-328. https://doi.org/10.1094/PDIS.1998.82.3.322
  3. Ben-Shalom, N. and Fallik, E. 2003. Further suppression of Botrytis cinerea disease in cucumber seedlings by chitosan-copper complex as compared with chitosan alone. Phytoparasitica 31:99-102. https://doi.org/10.1007/BF02979773
  4. Cho, S., Lee, S. H., Park, S., Choi, K. U., Cho, J., Hur, J. H., Shrestha, A. and Lim, C. 2009. Identification of a genetic locus related to antivirus production in Pseudomonas fluorescence strain Gpf01 against cucumber mosaic virus. Plant Pathol. J. 25:77-85. https://doi.org/10.5423/PPJ.2009.25.1.077
  5. Choi, G. J., Kim, J. C., Park, E. J., Choi, Y. H., Jang, K. S., Lim, H. K., Cho, K. Y. and Lee, S. W. 2006. Biological control activity of two isolates of Pseudomonas fluorescens against rice sheath blight. Plant Pathol. J. 22:289-294. https://doi.org/10.5423/PPJ.2006.22.3.289
  6. Cui, X. H. and Harling, R. 2006. Evaluation of bacterial antagonists for biological control of broccoli head rot caused by Pseudomonas fluorescens. Phytopathology 96:408-416. https://doi.org/10.1094/PHYTO-96-0408
  7. Darling, D., Harling, R., Simpson, R. A., McRoberts, N. and Hunter, E. A. 2000. Susceptibility of broccoli cultivars to bacterial head rot: in vitro screening and the role of head morphology in resistance. Eur. J. Plant Pathol. 106:11-17. https://doi.org/10.1023/A:1008759315557
  8. Devlieghere, F., Vermeulen, A. and Debevere, J. 2004. Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 21:703-714. https://doi.org/10.1016/j.fm.2004.02.008
  9. Fang, Y., Li, B., Wang, F., Liu, B. P., Wu, Z. Y., Su, T., Qiu, W. and Xie, G. L. 2009. Bacterial fruit rot of apricot caused by Burkholderia cepacia in China. Plant Pathol. J. 25:429-432. https://doi.org/10.5423/PPJ.2009.25.4.429
  10. Fujimoto, T., Tsuchiya, Y., Terao, M., Nakamura, N. and Yamamoto, M. 2006. Antibacterial effects of chitosan solution${\circledR}$ against Legionella pneumophila, Escherichia coli, and Staphylococcus aureus. Int. J. Food Microbiol. 112:96-101. https://doi.org/10.1016/j.ijfoodmicro.2006.06.003
  11. Hildebrand, P. D. 1989. Surfactant-like characteristics and identity of bacteria associated with broccoli head rot in Atlantic Canada. Can. J. Plant Pathol. 11:205-214. https://doi.org/10.1080/07060668909501101
  12. Ipper, N. S., Kim, J. E., Koo, J. H., Hur, J. H. and Lim, C. K. 2005. Inhibitory effects of a Korean strain Gpf01 identified as Pseudomonas fluorescens on cucumber mosaic virus. Plant Pathol. J. 21:262-269. https://doi.org/10.5423/PPJ.2005.21.3.262
  13. Kong, H. G., Choi, K. H., Heo, K. R., Lee, K. Y., Lee, H. J., Moon, B. J. and Lee, S. W. 2009. Generation of a constitutive green fluorescent protein expression construct to mark biocontrol bacteria using P43 promoter from Bacillus subtilis. Plant Pathol. J. 25:136-141. https://doi.org/10.5423/PPJ.2009.25.2.136
  14. Lee, Y. S., Han, H. S., Kim, G. H., Koh, Y. J., Hur, J. S. and Jung, J. S. 2009. Causal agents of blossom blight of Kiwifruit in Korea. Plant Pathol. J. 25:220-224. https://doi.org/10.5423/PPJ.2009.25.3.220
  15. Li, B., Wang, G. L., Wu, Z. Y., Qiu, W., Tang, Q. M. and Xie, G. L. 2009a. First report of bacterial head rot of broccoli caused by Pseudomonas fluorescens in China. Plant Dis. 93:1219.
  16. Li, B., Wang, X., Chen, R. X., Huangfu, W. G. and Xie, G. L. 2008b. Antibacterial activity of chitosan solution against Xanthomonas pathogenic bacteria isolated from Euphorbia pulcherrima. Carbohyd. Polym. 72:287-292. https://doi.org/10.1016/j.carbpol.2007.08.012
  17. Li, B., Xu, L. H., Lou, M. M., Li, F., Zhang, Y. D. and Xie, G. L. 2008a. Isolation and characterization of antagonistic bacteria against bacterial leaf spot of Euphorbia pulcherrima. Lett. Appl. Microbiol. 46:450-455. https://doi.org/10.1111/j.1472-765X.2008.02337.x
  18. Li, B., Yu, R. R., Yu, S. H., Qiu, W., Fang, Y. and Xie, G. L. 2009b. First report on bacterial heart rot of garlic caused by Pseudomonas fluorescens in China. Plant Pathol. J. 25:91-94. https://doi.org/10.5423/PPJ.2009.25.1.091
  19. Pajot, E. and Silue, D. 2005. Evidence that DL-3-aminobutyric acid and acibenzolar-S-methyl induce resistance against bacterial head rot disease of broccoli. Pest Manag. Sci. 61:1110-1114. https://doi.org/10.1002/ps.1103
  20. Photchanachai, S., Singkaew, J. and Thamthong, J. 2006. Effects of chitosan seed treatment on Colletotrichum sp. and seedlinggrowth of chili cv. “Jinda”. Acta Horticulturae 712:585-590.
  21. Saygili, H., Aysan, Y., Sahin, F., Ustun, N. and Mirik, M. 2004. Occurrence of pith necrosis caused by Pseudomonas fluorescens on tomato plants in Turkey. Plant Pathol. 53:803. https://doi.org/10.1111/j.1365-3059.2004.01092.x
  22. Seyfarth, F., Schliemann, S., Elsner, P. and Hipler, U. C. 2008. Antifungal effect of high- and low-molecular-weight chitosan hydrochloride, carboxymethyl chitosan, chitosan oligosaccharide and N-acetyl-d-glucosamine against Candida albicans, Candida krusei and Candida glabrata. Int. J. Pharm. 353:139-148.
  23. Su, Y. J. 2008. Spread and prospect of broccoli cultivar with high yield. Chin. Countryside Well-off Technol. 8:42-44.
  24. Vasanthi, H. R., Mukherjee, S. and Das, D. K. 2009. Potential health benefits of broccoli- a chemico-biological overview. Mini Rev. Med. Chem. 9:749-759. https://doi.org/10.2174/138955709788452685
  25. Wang, X. M., Cui, K. and Lu, Y. L. 2008. Overview of applicable value and production and export prospect of Chinese broccoli. Chin. Agri. Sci. Bull. 24:478-480.

Cited by

  1. Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot pathogen Burkholderia seminalis vol.346, pp.11, 2011, https://doi.org/10.1016/j.carres.2011.04.042
  2. Inhibitory effect and mode of action of chitosan solution against rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae RS-1 vol.391, 2014, https://doi.org/10.1016/j.carres.2014.02.025
  3. Effects of hydro-alcoholic extract of broccoli (Brassica oleracea) on sensory threshold of pain using the for-malin test in adult male rats vol.3, pp.7, 2014, https://doi.org/10.15412/J.JBTW.01030702
  4. Priming of plant resistance by natural compounds. Hexanoic acid as a model vol.5, 2014, https://doi.org/10.3389/fpls.2014.00488
  5. Evidence on antimicrobial properties and mode of action of a chitosan obtained from crustacean exoskeletons on Pseudomonas syringae pv. tomato DC3000 vol.97, pp.15, 2013, https://doi.org/10.1007/s00253-013-4993-8
  6. Effect of chitosan solution on the inhibition ofAcidovorax citrullicausing bacterial fruit blotch of watermelon vol.93, pp.5, 2013, https://doi.org/10.1002/jsfa.5812
  7. Chitosan for Eco-friendly Control of Plant Disease vol.11, pp.2, 2017, https://doi.org/10.3923/ajppaj.2017.53.70
  8. Nanochitosan supports growth of Zea mays and also maintains soil health following growth vol.7, pp.1, 2017, https://doi.org/10.1007/s13205-017-0668-y
  9. Synthesis, characterization, and antibacterial activity of chitosan/TiO 2 nanocomposite against Xanthomonas oryzae pv. oryzae vol.152, 2016, https://doi.org/10.1016/j.carbpol.2016.07.070
  10. Antibacterial activity of two chitosan solutions and their effect on rice bacterial leaf blight and leaf streak vol.69, pp.2, 2013, https://doi.org/10.1002/ps.3399
  11. Field efficacy of chitosan to control Pseudomonas syringae pv. actinidiae, the causal agent of kiwifruit bacterial canker vol.140, pp.4, 2014, https://doi.org/10.1007/s10658-014-0515-5
  12. Synthesis of chitosan based nanoparticles and their in vitro evaluation against phytopathogenic fungi vol.62, 2013, https://doi.org/10.1016/j.ijbiomac.2013.10.012
  13. Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde vol.11, pp.5, 2013, https://doi.org/10.3390/md11051534
  14. Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani vol.34, pp.12, 2012, https://doi.org/10.1007/s10529-012-1035-z
  15. Characterizing the mode of action of Brevibacillus laterosporus B4 for control of bacterial brown strip of rice caused by A. avenae subsp. avenae RS-1 vol.30, pp.2, 2014, https://doi.org/10.1007/s11274-013-1469-z
  16. Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima vol.17, pp.6, 2012, https://doi.org/10.3390/molecules17067028