- Volume 30 Issue 3
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Casein Phosphopeptide (CPP)-Producing Activity and Proteolytic Ability by Some Lactic Acid Bacteria
유산균의 Casein Phosphopeptide(CPP) 생산 및 단백질 분해 활성
- Cho, Yoon-Hee (Division of Animal Science, Chonnam National University) ;
- Oh, Se-Jong (Division of Animal Science, Chonnam National University)
- Received : 2010.01.05
- Accepted : 2010.05.11
- Published : 2010.06.30
Casein phosphopeptide (CPP) enhances calcium absorption in humans. Lactic acid bacteria (LAB) are capable of synthesis of cell-surface proteinase, which can hydrolyze milk protein and release several types of peptides in the medium. This study was conducted to characterize proteinase of LAB and to evaluate the CPP production from bovine milk. The content of CPP of milk produced by cell-free extract of LAB was determined based on the quantity of decomposed peptide from casein using the O-phthaldialdehyde (OPA) method. The proteolytic activity of LAB was assayed using fluorescein isothiocyanate (FITC)-labeled casein. Casein appeared to be a better substrate than whey proteins for extracellular proteinases of LAB. During fermentation, milk proteins were hydrolyzed by extracellular proteinase of LAB, resulting in an increase in the amount of free
casein phosphopeptide;casein phosphopeptide;lactic acid bacteria;proteolytic activity
Supported by : 농림기술관리센터
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