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Sericin Enhances Secretion of Thyroglobulin in the Thyrocytes

갑상선세포에서 sericin에 의한 thyroglobulin의 분비증가

  • Received : 2010.07.07
  • Accepted : 2010.07.15
  • Published : 2010.08.30

Abstract

Sericin is a type of high molecular weight water-soluble glycoprotein surrounding fibroin (silk protein) that has been used as a cell culture supplement and accelerates cell proliferation in various serum-free media. The purpose of this study was to investigate the enhancing effect of thyroglobulin (Tg) secretion by sericin in thyrocytes, FRTL-5 cells. While Tg-mRNA expression was not enhanced, a secreted form of Tg was obviously increased by sericin. In this status, expression of both endoplasmic reticulum (ER) molecular chaperones (Bip & calreticulin) and ER membrane proteins (IRE1, PERK & ATF6) was enhanced. The proximal step of IRE1, XBP1 mRNA splicing was slightly detected however, the proximal step of PERK, phosphorylation of $eIF2{\alpha}$, was changeless. In addition, sericin enhanced cell viability by the MTT assay. The above results showing the ability of sericin to promote protein production demonstrated its potential usefulness as a new biomaterial.

Keywords

Sericin;thyroglobulin;ER molecular chaperone

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  1. Processing and characterization of silk sericin from Bombyx mori and its application in biomaterials and biomedicines vol.61, 2016, https://doi.org/10.1016/j.msec.2015.12.082
  2. The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation vol.49, pp.6, 2017, https://doi.org/10.1007/s00726-017-2396-3