Effects of Dietary Supplementation of Fermented Garlic Powder on Immune Responses, Blood Components, and Disease Resistance against Principal Fish Disease of Juvenile Olive Flounder, Paralichthys olivaceus in Low Temperature Season

저수온기 넙치 치어에 있어서 발효마늘분말의 사료 내 첨가가 면역반응, 혈액성분 및 주요 어병세균에 대한 질병저항성에 미치는 영향

  • Kim, Sung-Sam (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Song, Jin-Woo (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lim, Se-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeong, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeon, You-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Yeo, In-Kyu (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lee, Kyeong-Jun (Faculty of Marine Biomedical Science, Jeju National University)
  • 김성삼 (제주대학교 해양의생명과학부) ;
  • 송진우 (제주대학교 해양의생명과학부) ;
  • 임세진 (제주대학교 해양의생명과학부) ;
  • 정준범 (제주대학교 해양의생명과학부) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • 여인규 (제주대학교 해양의생명과학부) ;
  • 이경준 (제주대학교 해양의생명과학부)
  • Received : 2010.04.20
  • Accepted : 2010.07.19
  • Published : 2010.08.31


We report non-specific immune responses and disease resistance against Vibrio anguillarum, Streptococcus iniae and Edwardsiella tarda by dietary supplementation of fermented garlic powder (FGP) in olive flounder for the first time. Four isonitrogenous (45% crude protein) and isocaloric (17.1 MJ/kg) diets were formulated to have 0%, 0.5%, 1% and 2% of the FGP (G-0, G-0.5, G-1 and G-2). The experimental diets were fed to juvenile olive flounder averaging 23.4 g in triplicate groups (90 fish/group) in a flow-through system. After a five-week feeding trial, healthy fish with similar sizes from each tank were selected and injected with 1 ml of three bacteria each to evaluate disease resistance of the fish. During the 5-week feeding trial, the weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio, and survival of the fish were not significantly affected by the experimental diets. However, feed intake was significantly lower (P<0.05) in the fish fed the G-2 diet compared with the control group. Hemoglobin, myeloperoxidase activity, cholesterol and HDL-cholesterol were not different between the dietary groups. However, hematocrit, nitroblue tetrazolium (NBT) activity, and lysozyme activity were increased (P<0.05) with an increment of dietary FGP. Plasma triglyceride of the fish fed the G-0.5 diet was significantly lower than that of fish fed the control diet. The cumulative mortality was lower in the fish fed diets containing FGP compared with the control group in the challenge test except for the bacteria Edwardsiella tarda. The results in this study indicate that dietary supplementation of FGP can enhance the non-specific immune responses and disease resistance of olive flounder against V. anguillarum and S. iniae.


Olive flounder;Fermented garlic powder;Non-specific immune response;Challenge test


Grant : 친환경 유기양식생산 매뉴얼 개발을 통한 양식넙치의 고품질화 및 가공기술 개발

Supported by : 한국연구재단


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