Effects of Aspergillus niger-Derived Multi-Enzyme Complex on Performance, Milk Yield, Blood Metabolites, and Manure Output in Multiparous Lactating Sows

사료내 Aspergillus niger 유래 복합효소의 첨가가 포유모돈의 생산성, 유생산, 혈액성상 및 분배설량에 미치는 영향

  • Lee, Sung-Hoon (Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute) ;
  • Ha, Young-Joo (Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute) ;
  • Kwack, Suk-Chun (Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute)
  • 이성훈 (경상남도 축산진흥연구소 축산시험장) ;
  • 하영주 (경상남도 축산진흥연구소 축산시험장) ;
  • 곽석준 (경상남도 축산진흥연구소 축산시험장)
  • Received : 2010.04.14
  • Accepted : 2010.08.04
  • Published : 2010.08.31


This study was conducted to investigate the effects of Aspergillus niger-derived multi-enzyme complex supplementation to feedrestricted lactating sows on performances, milk yield, blood profiles, and manure excretion as compared with ad libitum-fed sows without supplementation of enzyme. Fifty multiparous lactating Berkshire sows were allotted to 5 treatments of 10 sows per treatment during a 28-d lactation period and litter per sow was standardized to 9 suckling piglets. Treatments were ad libitum-fed sows without enzyme and feed-restricted sows supplemented with four increasing levels (0, 0.02, 0.04 and 0.08%) of multi-enzyme complex derived from Aspergillus niger. Blood samples from all sows were collected to determine serum metabolite concentrations before the morning feeding on d 27 of lactation. Litter body weight and a piglet weight at weaning, and litter weight gain significantly (P<0.05) increased with increasing levels of multi-enzyme complex, but there was no significant difference between ad libitum-fed sows without enzyme and feed-restricted sows supplemented with multi-enzyme complex. Body condition score and backfat depth at weaning significantly (P<0.05) increased as multi-enzyme complex level increased. Lactational backfat depth tended (P>0.05) to less decrease with increasing levels of enzyme complex. Serum inorganic phosphorus and non-esterified fatty acid concentrations significantly (P<0.05) increased with increasing levels of enzyme complex. Daily milk yield was not significantly different across treatments, but milk fat yield significantly (P<0.05) increased as multi-enzyme complex level increased. Manure output was significantly (P<0.01) higher for ad libitum-fed sows than for feed-restricted sows, but there was no significant difference among feed-restricted sows supplemented with increasing levels of multi-enzyme complex. Fecal phosphorus amount significantly (P<0.05) decreased with increasing levels of multi-enzyme complex. Feed costs of sows per litter weight gain were reduced by 1.25% to 9.67% with increasing levels of multi-enzyme complex as compared with ad libitum-fed sows without enzyme. The results indicated that multi-enzyme supplementation to feed-restricted lactating sows not only increased litter performances, but also was comparable to ad libitum-fed sows, resulting in reduced feed costs. Moreover, the reduction of fecal phosphorus amount with increasing levels of enzyme complex would contribute to the reduction of environmental pollution.


Lactating sows;Multi-enzyme complex;Litter weight gain;Milk fat yield;Fecal phosphorus


  1. AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists. Washington, DC.
  2. Boyd, R. D. and Kensinger, R. S. 1998. Metabolic precursors for milk synthesis. Page 71 in The Lactating Sow. Verstegen, M. W. A., Moughun, P. J. and Schrama, J. W. ed. Wageningen Press, Wageningen, the Netherlands.
  3. Brendemuhl, J. H., Lewis, A. J. and Peo Jr., E. R. 1987. Effect of protein and energy intake by primiparous sows during lactation on sow and litter performance and sow serum thyroxine and urea concentrations. J. Anim. Sci. 64:1060-1069.
  4. Coffey, R. D., Parker, G. R. and Laurent, K. M. 1999. Assessing sow body condition. In Publ. ASC 158, University of Kentucky Cooperative Extension Service, Lexington, KY.
  5. Eastham, P. R., Smith, W. C., Whittemore, C. T. and Phillips, P. 1988. Response of lactating sows to food level. Anim. Sci. 46:71-77.
  6. Fu, X., Zhu, X., Gao, K. and Duan, J. 1995. Oil and fat hydrolysis with lipase from Aspergillus sp. JAOCS 72:527-531.
  7. Harper, A. F., Kornegay, E. T. and Schell, T. C. 1997. Phytase supplementation of low-phosphorus growing-finishing pig diets improves performance, phosphorus digestibility, and bone mineralization and reduces phosphorus excretion. J. Anim. Sci. 75:3174-3186.
  8. Ji, F. and Kim, S. W. 2004. Effects of carbohydrase supplement on lactation performance of primiparous sows fed corn-soybean meal based lactation diet. Asian-Aust. J. Anim. Sci. 17:533-537.
  9. King, R. H. and Williams, I. H. 1984a. The effect of nutrition on the reproductive performance of first-litter sows. 1. Feeding level during lactation, and between weaning to mating. Anim. Prod. 38:241-247.
  10. King, R. H. and Williams, I. H. 1984b. The effect of nutrition on the reproductive performance of first-litter sows. 2. Protein and energy intakes during lactation. Anim. Prod. 38:249-325.
  11. Kirkwood, R. N., Baidoo, S. K., Aherne, F. X. and Sather, A. S. 1987. The influence of feeding level during lactation on the occurrence and endocrinology of the post weaning estrus in sows. Can. J. Anim. Sci. 67:405-415.
  12. Koketsu, Y., Dial, G. D., Pettigrew, J. E. and King, V. L. 1997. Influence of feed intake during individual weeks of lactation on reproductive performance of sows on commercial farms. Livest. Prod. Sci. 49:217-225.
  13. Lewis, G. E., Sanchez, W. K., Treacher, R., Hunt, C. W. and Pritchard, G. T. 1995. Effect of direct-fed fibrolytic enzymes on lactational performance of midlactation Holstein cows. Proc. West. Sect. Am. Soc. Anim. Sci. Can. Soc. Anim. Sci. 46:310-313.
  14. Macris, J. B., Kourentzi, E. and Hatzinkolaou, D. G. 1996. Studies on localization and regulation of lipase production by Aspergillus niger. Process Biochem. 31:807-812.
  15. Mahan, D. C. and Mangan, L. T. 1975. Evaluation of various protein sequences on the nutritional carry-over from gestation to lactation with first-litter sows. J. Nutr. 105:1291-1298.
  16. Mullan, B. P. and Williams, I. H. 1989. The effect of body reserves at farrowing on the reproductive performance of first-litter sows. Anim. Prod. 48:449-457.
  17. NCR-42 Committee Swine Nutrition. 1978. Effect of protein level during gestation and lactation on reproductive performance in swine. J. Anim. Sci. 46:1673-1684.
  18. Nyachoti, C. M., Arntfield, S. D., Guenter, W., Cenkowski, S. and Opapeju, F. O. 2006. Effect of micronized pea and enzyme supplementation on nutrient utilization and manure output in growing pigs. J. Anim. Sci. 84:2150-2156.
  19. O’Connell, J. M., Callan, J. J. and O’Doherty, J. V. 2006. The effect of dietary crude protein level, cereal type and exogenous enzyme supplementation on nutrient digestibility, nitrogen excretion, faecal volatile fatty acid concentration and ammonia emissions from pigs. Anim. Feed Sci. Technol. 127:73-88.
  20. Omogbenigun, F. O., Nyachoti, C. M. and Slominski, B. A. 2004. Dietary supplementation with multi-enzyme preparations improves nutrient utilization and growth performance in weaned pigs. J. Anim. Sci. 82:1053-1061.
  21. Pluske, J. R., Williams, I. H., Zak, L. J., Clowes, E. J., Cegielski, A. C. and Aherne, F. X. 1998. Feeding lactating primiparous sows to establish three divergent metabolic states: III. Milk production and pig growth. J. Anim. Sci. 76:1165-1171.
  22. Pointillart, A., Fontaine, N. and Thomasset, M. 1984. Phytate phosphorus utilization and intestinal phytases in pigs fed low phosphorus: Wheat or corn diets. Nutr. Rep. Int. 29:473-483.
  23. Pointillart, A., Fourdin, A. and Fontaine, N. 1987. Importance of cereal phytase activity for phytate phosphorus utilization by growing pigs fed diets containing triticale or corn. J. Nutr. 117:907-913.
  24. Prunier, A., Dourmad, J. Y. and Etienne, M. 1993. Feeding level, metabolic parameters and reproductive performance of primiparous sows. Livest. Prod. Sci. 37:185-196.
  25. Reese, D. E., Moser, B. D., Peo Jr., E. R., Lewis, A. J., Zimmerman, D. R., Kinder, J. E. and Stroup, W. W. 1982. Influence of energy intake during lactation on the interval from weaning to first estrus in sows. J. Anim. Sci. 55:590-598.
  26. SAS. 2009. SAS/$STAT^{(R)}$ User's guide (Release 9.1 ed.). Statistics, SAS Inst, Inc., Cary, NC.
  27. Speer, V. C. and Cox, D. F. 1984. Estimating milk yield of sows. J. Anim. Sci. 59:1281-1285.
  28. Veum, T. L. and Ellersieck, M. R. 2008. Effect of low doses of Aspergillus niger phytase on growth performance, bone strength, and nutrient absorption and excretion by growing and finishing swine fed corn-soybean meal diets deficient in available phosphorus and calcium. J. Anim. Sci. 86:858-870.
  29. Yang, W. Z., Beauchemin, K. A. and Rode, L. M. 1999. Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82:391-403.
  30. Zak, L. J., Cosgrove, J. R., Aherne, F. X. and Foxcroft, G. R. 1997. Pattern of feed intake and associated metabolic and endocrine changes differentially affect postweaning fertility in primiparous lactating sows. J. Anim. Sci. 75:208-216.
  31. 김효진, 조진호, 진영걸, 유종상, 신승오, 황염, 김인호. 2007. 포유모돈에 있어 복합효소제 함유 식물성 단백질 공급원이 생산성과 돈유성상에 미치는 영향. 한국동물자원과학회지. 49:745-752.
  32. 통계청. 2010. 2009년 축산물생산비 조사결과.
  33. 한국육류유통수출입협회. 2010. 육류 유통 실태조사. VOL11 p. 79-80.