Bacterial contaminants in extended boar semen and selection of effective antimicrobials

돼지 희석정액의 세균오염도 및 유효 항생제 선발

  • Kim, Ha-Young (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Byun, Jae-Won (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Shin, Dong-Ho (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Kim, Hyoung-Soon (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Yoon, Hachung (Veterinary Epidemiology Division, National Veterinary Research and Quarantine Service) ;
  • Park, Choi-Kyu (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Lee, O-Soo (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Jung, Byeong Yeal (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service)
  • 김하영 (국립수의과학검역원 질병진단센터) ;
  • 변재원 (국립수의과학검역원 질병진단센터) ;
  • 신동호 (국립수의과학검역원 질병진단센터) ;
  • 김형순 (국립수의과학검역원 질병진단센터) ;
  • 윤하정 (역학조사과) ;
  • 박최규 (국립수의과학검역원 질병진단센터) ;
  • 이오수 (국립수의과학검역원 질병진단센터) ;
  • 정병열 (국립수의과학검역원 질병진단센터)
  • Accepted : 2010.05.12
  • Published : 2010.06.30

Abstract

Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups $(\leq\;24\;and\;>\;24\;month\;old).$ Stenotrophomonas maltophilia (n = 18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n = 12), Sphingomonas paucimobilis (n = 12), Myroides spp. (n = 5), Ochrobactrum anthropi (n = 3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.

Acknowledgement

Supported by : 국립수의과학검역원

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