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G1 Arrest of the Cell Cycle by Gomisin N, a Dibenzocyclooctadiene Lignan, Isolated from Schizandra chinensis Baill in Human Leukemia U937 Cells

오미자에서 분리된 dibenzocyclooctadiene lignan의 일종인 gomisin N에 의한 인체혈구암세포의 세포주기 G1 arrest 유발

  • Park, Cheol (Blue-Bio Industry Regional Innovation Center, Dongeui University) ;
  • Hwang, Hye-Jin (Department of Food and Nutrition, College of Human Ecology, Dongeui University) ;
  • Choi, Byung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Choi, Tae-Hyun (Department of Ophthalmic Optics, Daegu Technical University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, College of Natural Sciences, Dongeui University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, Pusan National University) ;
  • Choi, Yung-Hyun (Department of Biomaterial Control, Graduate School, Dongeui University)
  • 박철 (동의대학교 블루바이오소재개발센터) ;
  • 황혜진 (동의대학교 생활과학대학 식품영양학과) ;
  • 최병태 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 최태현 (대구공업대학 안경광학과) ;
  • 김병우 (동의대학교 자연과학대학 응용생물학과) ;
  • 최영환 (부산대학교 생명자원과학대학 원예학과) ;
  • 최영현 (동의대학교 대학원 바이오물질제어학과)
  • Received : 2010.03.23
  • Accepted : 2010.07.15
  • Published : 2010.07.30

Abstract

We investigated the anti-cancer effects of two dibenzocyclooctadiene lignans, gomisin A and gomisin N, isolated from Schizandra chinensis Baill, in human promyelocytic U937 cells. Gomisin N, but not gomisin A, inhibited cell growth in a concentration-dependent manner, which was associated with the induction of G1 arrest of the cell cycle. G1 arrest induced by gomisin N was correlated with down-regulation of cyclin E, cyclin-dependent kinase (Cdk) 2 and Cdk4, and a concomitant up-regulation of Cdk inhibitors such as p16 (INK4A) and p21 (WAF1/CIP1). Furthermore, gomisin N inhibited phosphorylation of retinoblastoma protein (pRB) and p130, and expression of transcription factor E2Fs. The results indicated that growth inhibition by gomisin N is related to cell cycle arrest at G1 in U937 cells and these findings suggest that gomisin N may be a useful chemotherapeutic agent.

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