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Protective Effect of Enzymatic Extracts from Sargassum coreanum on H2O2-induced Cell Damage

  • Ko, Seok-Chun (Department of Marine Life Science, Jeju National University) ;
  • Kang, Sung-Myung (Department of Marine Life Science, Jeju National University) ;
  • Lee, Seung-Hong (Department of Marine Life Science, Jeju National University) ;
  • Ahn, Gin-Nae (Department of Marine Life Science, Jeju National University) ;
  • Kim, Kil-Nam (Jeju Biodiversity Research Institute (JBRI) and Jeju Hi-Tech Industry Development Institute (HiDI)) ;
  • Kim, Yong-Tae (Department of Food Science and Biotechnololgy, Kunsan National University) ;
  • Kim, Jin-Soo (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min-Soo (Department of Food Science and Nutrition/Institute of Marine Industry, Gyeongsang National University) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
  • Received : 2010.01.19
  • Accepted : 2010.03.15
  • Published : 2010.03.31

Abstract

In our previous study, we preliminarily demonstrated that Celluclast and Neutrase extracts exhibited the strongest $H_2O_2$-scavenging activities among five carbohydrases (Viscozyme, Celluclast, Termamyl, Ultraflo and AMG) and five proteases (Kojizyme, Alcalse, Flavourzyme Protamex and Neutrase) extracts. Thus, Celluclast and Neutrase extracts were selected for use in further experiments and were separated into four different molecular weight fractions (<5, 5-10, 10-30 and >30 kDa). Among them, the 5-10 kDa fraction showed the highest $H_2O_2$-scavenging activity. The 5-10 kDa fraction also strongly enhanced cell viability against $H_2O_2$-induced oxidative damage. Furthermore, the fraction reduced the proportion of apoptotic cells induced by $H_2O_2$, as demonstrated by decreased sub-G1 hypodiploid cells and decreased apoptotic body formation by flow cytometry. These results indicated that the 5-10 kDa fraction of the Celluclast and Neutrase extracts from S. coreanum exhibited strong antioxidant activity over $H_2O_2$-mediated cell damage in vitro.

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