The Effect of Microcurrent Stimulation on Expression of BMP-4 After Tibia Fracture in Rabbits

미세전류가 토끼 경골의 골절 후 BMP-4 발현에 미치는 영향

  • Received : 2009.08.26
  • Accepted : 2009.10.21
  • Published : 2010.03.28


This study aimed to examine the effect of microcurrent stimulation on expression of Bone Morphogenetic Protein(BMP) 4 after tibia fracture in rabbits. The twenty four adult 6 month old New Zealand white rabbits weighting 2.5~3.5 ㎏ were used. Twenty four rabbits with tibia fracture were randomly divided into control and experimental groups. Each group was divided into four subgroups, based on the duration of the experiment (3, 7, 14, 28 days). The experimental groups received microcurrent stimulation of 20~25 ${\mu}A$ intensity with surface Ag-AgCl electrode (diameter 1cm, Biopac, U.S.A.) for 24 hours a day. Cathode of the microcurrent stimulator located on the tibia directly, anode of it did on the gastrocnemius muscle. After evaluation, the test results are as follows: Comparisons of immunohistochemical observation of BMP-4 in 7 days after tibial fracture show that there was shown to be a moderate positive reaction (++) on concentric circles of Harversian system and the interstitial lamella in the control group, while there was a very strong positive reaction () on concentric circles of Harversian system and interstitial lamellain the experimental group. These results suggest that applying non-invasive constant microcurrent stimulation on fractured bone is helpful to bone healing.


  1. A. E, Karamitros, V. N, Kalentzos, and P. N. Soucacos, "Electric stimulation and hyperbaric oxygen therapy in the treatment of nonunions," Injury, Vol.37, No.S, pp.63-73, 2006.
  2. M. P, Bostrom, J. M, Lane, W. S, and Berberian, "Immunolocalization and expression of bone morphogenetic proteins2 and 4 in fracture healing," J Orthop Res, Vol.13, No.3, pp.357-367, 1995.
  3. M. L, Bouxsein, T. J, Turek, and C. A, Blakel, "Recombinant human bone morphogenetic protein-2 accelerates healing in a rabbit ulnar osteotomy model," J Bone Joint Surg Am, Vol.83-A, No.8, pp.881219-1230, 2001.
  4. J. M, Granjeiro, R. C, Oliveira, and J. C, Bustos-Valenzuela, "Bone morphogenetic proteins: from structre to clinical use," Br J Med Biol Res, Vol.38, No.10, pp.1463-1473, 2005.
  5. S, Croteau, F, Rauch, and A, Silvestri, "Bone morphogenetic proteins in orthopedics: from basic science to clinical practices," Orthopedics, Vol.22, No.7, pp.686-695, 1999.
  6. R. A, Kanaan and L. A. Kanaan, "Transforming growth gactor ${\beta}$ 1, bone connection," Med Med Sci Monit, Vol.12, No.8, pp.164-169, 2006.
  7. S, Suttapreyasri, S, Koontongkaew, and A, Phongdara, "Expression of bone morphogenetic protiens in normal human intramembranous and endochondral bones," Int J Oral Maxillofac Surg, Vol.35, No.5, pp.444-452, 2006.
  8. M, Egermann, C. A, Lill, and K, Griesbeck, "Effects of BMP-2 gene transfer on bone healing in sheep," Gene Therapy, Vol.13, No.17, pp.1290-1299, 2006.
  9. R. H, Li, M. L, Bouxsein, and C. A, Blake, "rhBMP-2 injected in a calcium phosphate paste(${\alpha}$-BSM) accelerates healing in the rabbit ulnar osteotomy model," J. J al Ortho Res, Vol.21, No.6, pp.997-1004, 2003.
  10. 박래준, “경피신경자극과 미세전류자극이 정상인의 교감신경 긴장도에 미치는 영향”, 대한물리치료학회지, Vol9, No.1, pp.51-57, 1997.
  11. J. A, Spadaro and R. O. Becker, "Function of implanted cathodes in electrode-induced bone Growth," Med. Biol. Eng Comput, Vol.17, No.6, pp.769-775, 1979.
  12. T. J Jr, Baranowski, J, Black, and C. T, Brighton, "Electrical osteogenesis by low direct current," J Orthop Res, Vol.1, No.2, pp.120-128, 1983.
  13. C. T, Brighton, W, Wang, and R, Seldes, "Signal transduction in electrically stimulated bone cells," J Bone Joint Surg Am, Vol.83-A, No.10, pp.1514-1523, 2001.
  14. 이덕용, 정문상, 성상철, 김명호, “전기자극 기간이 신생골 형성에 미치는 영향”, 대한정형외과학회지, Vol.19, No.5, pp.757-764, 1984.
  15. T, Noshi, T, Yoshikawa, and Y, Dohi, "Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composities," Artif Organs, Vol.25, No.3, pp.201-208, 2001.
  16. Y, Shimakura, Y, Yamzaki, and E. Uchinuma, "Experimental study on bone formation potential of cryopreserved human bone marrow mesenchymal cell/hydroxyapatite complex in the presence of recombinant human bone morphogenetic protein-2," J Craniofac Surg, Vol.14, No.1, pp.108-116 2003.
  17. T, Onishi, Y, Ishidou, and T, Nagamine, "Distinct and overlapping patterns of localization of bone morphogenetic protein (BMP) family members and a BMP type II receptor during fracture healing in rats," Bone, Vol.22, No.6, pp.605-612, 1998.
  18. M. Nakashima, "Mitogenic and dentin inductive effects of crude bone morphogenetic protein from bone and dentin in primary adult pulp cell culture," Oral Surg Oral Med Oral Patho, Vol.73, No.4, pp.484-489, 1992.
  19. M, Ronga, F, Baldo, and G, Zappala, "Recombinant human bone morphogenetic protein-7 for treatment of long bone non-union: An observational, retrospective, non-randomized study of 105 patients," Injury, Vol.37, No.Suppl 3, pp.S51-S56, 2006.
  20. J, Park, R, Lutz, E, and Felszeghy, "The effect on bone regeneration of a liposomal vector to deliver BMP-2 gene to bone grafts in peri-implant bone defects," Biomaterials, Vol.28, No.17, pp.2772-2782, 2007.

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