Molecular Monitoring of Plankton Diversity in the Seonakdong River and Along the Coast of Namhae

분자 모니터링을 이용한 서낙동강과 남해 연안 플랑크톤 군집 분석

  • Kim, Bo-Kyung (Division of Earth Environmental System, Pusan National University) ;
  • Lee, Sang-Rae (Marine Research Institute, Pusan National University) ;
  • Lee, Jin-Ae (School of Environmental Science and Engineering, Inje University) ;
  • Chung, Ik-Kyo (Division of Earth Environmental System, Pusan National University)
  • 김보경 (부산대학교 지구환경시스템학부) ;
  • 이상래 (부산대학교 해양연구소) ;
  • 이진애 (인제대학교 환경공학부) ;
  • 정익교 (부산대학교 지구환경시스템학부)
  • Received : 2010.02.14
  • Accepted : 2010.02.26
  • Published : 2010.02.28

Abstract

The biodiversity of eukaryotic plankton has commonly been used to evaluate the status of aquatic ecosystems. Therefore, an accurate and rapid method for species identification is needed to reveal the biodiversity of environmental water samples. To date, molecular methods have provided a great deal of information that has enabled identification of the hidden biodiversity in environmental samples. In this study, we utilized environmental polymerase chain reaction (PCR) and constructed the 18S nuclear ribosomal RNA clone library from environmental water samples in order to develop more efficient methods for species identification. For the molecular analysis, water samples were collected from the Seonakdong River (Gimhae Bridge) and the coast of Namhae,(Namhaedo). Colony PCR and restriction fragment length polymorphism of PCR (PCR-RFLP) were then adopted to isolate unique clones from the 18S rDNA clone library. Restriction fragment length polymorphism pattern analysis of the Gimhae Bridge sample revealed 44 unique clones from a total of 60 randomly selected clones, while analysis of the Namhae sample revealed 27 unique clones from 150 clones selected at random. A BLAST search and subsequent phylogenetic analysis conducted using the sequences of these clones revealed hidden biodiversity containing a wide range of taxonomic groups (Heterokontophyta (7), Ciliophora (23), Dinophyta (1), Chytridiomycota (1), Rotifera (1) and Arthropoda (11) in the Gimhae Bridge samples Ciliophora (4), Dinophyta (3), Cryptophyta (1), Arthropoda (19) in the Namhae samples). Therefore, the molecular monitoring method developed here can provide additional information regarding the biodiversity and community structure of eukaryotic plankton in environmental samples and helps construct a useful database of biodiversity for aquatic ecosystems.

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