Immunoregulatory Effects of Water Extracts of Inonotus obliquus in Carbon Tetrachloride-Induced Liver Damage Animal Model

사염화탄소로 유도된 간 손상 동물모델에서 차가버섯 열수 추출물이 면역 조절 기능에 미치는 영향

  • An, Chi-Sun (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Jin, Hai-Lan (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Jeon, Yun-Hui (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Bak, Jong-Phil (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Kim, Jong-Dai (Medical & Bio-material Research Center, Kangwon University) ;
  • Yoon, Jung-Han (Medical & Bio-material Research Center, Kangwon University) ;
  • Lim, Beong-Ou (College of Biomedical & Health Science, Department of Life Science, Konkuk University)
  • 안치선 (건국대학교 의료생명대학 생명과학부) ;
  • 김해란 (건국대학교 의료생명대학 생명과학부) ;
  • 전윤희 (건국대학교 의료생명대학 생명과학부) ;
  • 박종필 (건국대학교 의료생명대학 생명과학부) ;
  • 김종대 (강원대학교 의료.바이오신소재융복합연구사업단) ;
  • 윤정한 (강원대학교 의료.바이오신소재융복합연구사업단) ;
  • 임병우 (건국대학교 의료생명대학 생명과학부)
  • Received : 2009.08.27
  • Accepted : 2009.12.30
  • Published : 2010.02.28

Abstract

Inonotus obliquus is one of the immune-regulatory substances and is recognized to play the role in the metabolic process of inflammation, allergy and immuntiy. The purpose of this study was to evaluate the effects of water extracts of Inonotus obliquus (IOW) on the liver lymphocyte immune function in the Sprague-Dawley male rats treated with carbon tetrachloride ($CCl_4$) to induce liver damage. Rats were fed with each experimental diet and water for 4 weeks. We found that effects of IOW on interferon-gamma (IFN-$\gamma$), signal transducer and activator of transcription 1 (STAT1), phospho-signal transducer and activator of transcription 1 (pSTAT1) and GATA-binding protein 3 (GATA-3) were decrease in vivo. Interleukin-4 (IL-4), STAT6, pSTAT6 and T-box expressed in T-cells (T-bet) decreased significantly lower in $CCl_4$+IOW group than the $CCl_4$ group. Our data indicated that cytokine protein production were increased in $CCl_4$ group and $CCl_4$+IOW group. As a result of this study, we assume that IOW fed could regulate the immuno-modulating functions through regulate the cytokine production capacity activated by liver damage.

Acknowledgement

Supported by : 교육과학기술부, 한국산업기술재단

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