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Effect of Sulforaphane on LPS-Induced Matrix Metalloproteinase-9 (MMP-9) Expression

Sulfolaphane이 lipopolysaccharide (LPS)에 의해 유도된 matrix metalloproteinase-9 (MMP-9) 발현에 미치는 영향

  • Lee, Jung-Tae (Department of Immunology, School of Medicine, Keimyung University) ;
  • Woo, Kyung-Jin (Department of Immunology, School of Medicine, Keimyung University) ;
  • Kwon, Taeg-Kyu (Department of Immunology, School of Medicine, Keimyung University)
  • 이정태 (계명대학교 의과대학 면역학 교실) ;
  • 우경진 (계명대학교 의과대학 면역학 교실) ;
  • 권택규 (계명대학교 의과대학 면역학 교실)
  • Received : 2010.01.05
  • Accepted : 2010.02.22
  • Published : 2010.02.28

Abstract

Sulforaphane is a naturally occurring member of the iosothiocyanate family, which reveals chemopreventive capacities including anti-cancer, anti-inflammation and inhibition of MMP-9 activities. In this study, we investigated the effect of sulforaphane on the expression of matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced Raw 264.7 cells. Sulforaphane strikingly suppressed the LPS-induced MMP-9 activity and mRNA expression in a dose-dependent manner. In addition, sulforaphane inhibited not only the LPS-induced MMP-9 promoter activity but also LPS-mediated activator protein-1 (AP-1) and nuclear factor-kB (NF-${\kappa}B$) promoter activity. Transient transfection by MMP-9 constructs, in which specific transcriptional factors were mutagenized, indicated that the effects of LPS and sulforaphane were mediated via AP-1 and NF-${\kappa}B$ response elements. We found that sulforaphane had the ability to suppress LPS-induced invasion in vitro. Taken together, these results demonstrated that sulforaphane effectively suppressed LPS-induced MMP-9 expression via modulation of promoter elements (AP-1 and NF-${\kappa}B$) in MMP-9 transcriptional activation.

Acknowledgement

Supported by : 한국과학재단

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