Effect of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-tang) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice

익기보혈탕(益氣補血湯)이 C57BL/6 마우스의 육모(育毛)촉진 및 Cytokine 변화에 미치는 영향

  • Hong, Jung-Ae (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Song, Mi-Yeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Choi, In-Hwa (Dept. of Oriental Ophthalmology & Otolaryngology & dermatology, College of Oriental Medicine, Kyunghee University) ;
  • Sohn, Nak-Won (Dept. of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung-Hee University) ;
  • Chung, Seok-Hee (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University)
  • 홍정애 (경희대학교 한의과대학 한방재활의학교실) ;
  • 송미연 (경희대학교 한의과대학 한방재활의학교실) ;
  • 최인화 (경희대학교 한의과대학 안이비인후피부과학교실) ;
  • 손락원 (경희대학교 동서의학대학원 신경과학 및 뇌질환) ;
  • 정석희 (경희대학교 한의과대학 한방재활의학교실)
  • Received : 2009.08.27
  • Accepted : 2009.10.28
  • Published : 2010.01.30

Abstract

Objective: Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) on hair regrowth and cytokine changes in a shaving model of C57BL6 mice. Method: Five-week-old mice were acclimated for 1 week at a temperature between $21-23^{\circ}C{\acute{E}}$, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) extract (sample) in 18 female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF-${\beta}1$ and EGF were also determined using immunohistochemistry. In addition to that, IFN-$\gamma$, IL-4 and IL-10 were determined in serum. Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF-${\beta}1$ was not revealed in either control or sample group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN-$\gamma$ was significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance. Conclusion: These results suggest that Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN-$\gamma$, and an increase of serum IL-4.

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