Microbial Conversion of Ginsenoside from the Extract of Korean Red Ginseng (Panax ginseng) by Lactobacillus sp.

  • Cho, Hye-Jin (Department of Food and Nutrition, Korea University) ;
  • Jung, Eun-Young (Department of Food and Nutrition, Korea University) ;
  • Oh, Sung-Hoon (Department of Food and Biotechnology, Ansan College of Technology) ;
  • Yoon, Brian (Sejong Corporation) ;
  • Suh, Hyung-Joo (Department of Food and Nutrition, Korea University) ;
  • Lee, Hyun-Sun (Department of Food and Nutrition, Korea University)
  • Received : 2010.05.01
  • Accepted : 2010.06.22
  • Published : 2010.06.30


Thirty-four strains of Lactobacillus species were isolated from soil and eight of these isolates (M1-4 and P1-4) were capable of growing on red ginseng agar. The M1 and P2 strains were determined to be L. plantarum and other strains (M2, M3, M4, P1, P3 and P4) were determined to be L. brevis. Fermentation of red ginseng extract (RGE) with strains M1, M2, P2 and P4 resulted in a low level of total carbohydrate content (174.3, 170.0, 158.8 and 164.8 mg/mL, respectively). RGE fermented by M3 showed a higher level of uronic acid than the control. The polyphenol levels in RGE fermented by M1, P1 and P2 (964.9, 941.7 and $965.3\;{\mu}g/mL$, respectively) were higher than the control ($936.8\;{\mu}g/mL$). Total saponin contents in fermented RGE (except M1) were higher than the control. RGE fermented by M2 and M3 had the highest levels of total ginsenosides (31.7 and 32.7 mg/mL, respectively). The levels of the ginsenoside Rg3 increased from 2.6 mg/mL (control) to 3.0 mg/mL (M2) or 3.1 mg/mL (M3). RGE fermented by M2 and M3 also had the highest levels of Rg5+Rk1 (7.7 and 8.3 mg/mL, respectively). Metabolite contents of ginsenoside (sum of CK, Rh1, Rg5, Rk1, Rg3 and Rg2) of M2 (13.0 mg/mL) and M3 (13.9 mg/mL) were also at a high level among the fermented RGE. Protopanaxadiol and protopanaxatriol content of ginsenoside of M2 (10.9 and 5.4 mg/mL, respectively) and M3 (11.0 and 5.7 mg/mL, respectively) were at higher levels than other fermented RGE.


  1. Nagai M, Ando T, Tanaka N, Tanaka O, Shibata S. 1972.Chemical studies on the oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng: Stereochemistry of the sapogenin of ginsenosides-Rb1,-Rb2 and -Rc. Chem Pharm Bull 20: 1212-1216.
  2. Shibata S, Ando T, Tanaka O. 1966. Chemical studies on the oriental plant drugs. XVII. The prosapogenin of the ginseng saponins (ginsenosides -Rb1, -Rb2, and -Rc). Chem Pharm Bull (Tokyo) 14: 1157-1161.
  3. Shibata S, Fujita M, Itokawa H, Tanaka O, Ishii T. 1963.Studies on the constituents of Japanese and Chinese crude drugs. Xi. Panaxadiol, a sapogenin of ginseng roots. Chem Pharm Bull (Tokyo) 11: 759-761.
  4. Park J, Cho JY. 2009. Anti-inflammatory effects of ginsenosides from Panax ginseng and their structural analogs.Afr J Biotechnol 8: 3682-3690.
  5. Mochizuki M, Yoo YC, Matsuzawa K, Sato, K. Saiki I,Tonooka S, Samukawa K, Azuma I. 1995. Inhibitory effect of tumor-metastasis in mice by saponins, ginsenoside-Rb2, 20(R)-ginsenoside-Rg3 and 20(S)-ginsenoside-Rg3, of redginseng. Biol Pharm Bull 18: 1197-1202.
  6. Wu JY, Gardner BH, Murphy CI, Seals JR, Kensil CR,Recchia J, Beltz GA, Newman GW, Newman MJ. 1992.Saponin adjuvant enhancement of antigen-specific immune-responses to an experimental Hiv-1 vaccine. J Immunol 148: 1519-1525.
  7. Wu JY, Gardner BH, Murphy CI, Seals JR, Kensil CR,Recchia J, Beltz GA, Newman GW, Newman MJ. 1992.Saponin adjuvant enhancement of antigen-specific immune-responses to an experimental Hiv-1 vaccine. J Immunol 148: 1519-1525.
  8. Popovich DG, Kitts DD. 2002. Structure-function relationship exists for ginsenosides in reducing cell proliferation and inducing apoptosis in the human leukemia (THP-1) cell line. Arch Biochem Biophys 406: 1-8.
  9. Sato K, Mochizuki M, Saiki I, Yoo YC, Samukawa K,Azuma I. 1994. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2. Biol Pharm Bull 17: 635-639.
  10. Sato K, Mochizuki M, Saiki I, Yoo YC, Samukawa K,Azuma I. 1994. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2. Biol Pharm Bull 17: 635-639.
  11. Noh KH, Son JW, Kim HJ, Oh DK. 2009. Ginsenoside compound K production from ginseng root extract by a thermostable beta-glycosidase from sulfolobus solfataricus. Biosci Biotechnol Biochem 73: 316-321.
  12. Karikura M, Miyase T, Tanizawa H, Taniyama T, TakinoY. 1991. Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside-Rb1 and -Rb2 in the digestive tract of rats. Chem Pharm Bull (Tokyo) 39: 2357-2361.
  13. Tawab MA, Bahr U, Karas M, Wurglics M, Schubert-Zsilavecz M. 2003. Degradation of ginsenosides in humans after oral administration. Drug Metabo Dispos 31:1065-1071.
  14. Hasegawa H. 2004. Proof of the mysterious efficacy of ginseng: Basic and clinical trials: Metabolic activation of ginsenoside: Deglycosylation by intestinal bacteria and esterification with fatty acid. J Pharmacol Sci 95: 153-157.
  15. Han BH, Park MH, Han YN, Woo LK, Sankawa U, YaharaS, Tanaka O. 1982. Degradation of ginseng saponins under mild acidic conditions. Planta Med 44: 146-149.
  16. Ko SR, Suzuki Y, Choi KJ, Kim YH. 2000. Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside Rh1, from ginsenosides Re and Rg1. Biosci Biotechnol Biochem 64: 2739-2743.
  17. Ko YJ, Park SH, Park BC, Lee Y-H, Kim J-A. 2007.Fermented ginseng with Bifidobacterium inhibits angiogenesisof human umbilical endothelial cells in vitro andin vivo. J Appl Pharmacol 15: 89-94.
  18. Bae EA, Hyun YJ, Choo MK, Oh JK, Ryu JH, Kim DH.2004. Protective effect of fermented red ginseng on a transientfocal ischemic rats. Arch Pharm Res 27: 1136-1140.
  19. Bae EA, Han MJ, Choo MK, Park SY, Kim DH. 2002. Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities. Biol Pharm Bull 25: 58-63.
  20. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406-425.
  21. Ando T, Tanaka O, Shibata S. 1971. Chemical studies on the oriental plant drugs (XXV). Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Shoyakuga Zasshi 25: 28-24.
  22. Kim SJ, Murthy HN, Hahn EJ, Lee HL, Paek KY. 2007.Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng (Panax ginseng C.A. Meyer). Sep Purif Technol 56: 401-406.
  23. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999.Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent.Method Enzymol 299: 152-178.
  24. Dubois M, Gilles K, Hamilton JK, Rebers PA, Smith F.1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356
  25. Blumenkr N, Asboehan G. 1973. New method for quantitative-determination of uronic acids. Anal Biochem 54:484-489.
  26. Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7: 1085-1097.
  27. Sato K, Yoo YC, Mochizuki M, Saiki I, Takahashi TA,Azuma I. 1995. Inhibition of tumor-induced angiogenesis by a synthetic lipid A analogue with low endotoxicity, DT-5461. Jpn J Cancer Res 86: 374-382.
  28. Kim PI, Jung MY, Chang YH, Kim S, Kim SJ, Park YH.2007. Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract.Appl Microbiol Biotechnol 74: 1103-1111.
  29. Bae EA, Park SY, Kim DH. 2000. Constitutive beta-glucosidases hydrolyzing ginsenoside Rb1 and Rb2 from human intestinal bacteria. Biol Pharm Bull 23: 1481-1485.
  30. Park SY, Bae EA, Sung JH, Lee SK, Kim DH. 2001. Purification and characterization of ginsenoside Rb1- metabolizing beta-glucosidase from Fusobacterium K-60, a human intestinal anaerobic bacterium. Biosci BiotechnolBiochem 65: 1163-1169.
  31. Dong A, Ye M, Guo H, Zheng J, Guo D. 2003. Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata. Biotechnol Lett 25: 339-344.
  32. Coskun H, Ondul E. 2004. Free fatty acid accumulation by mesophilic lactic acid bacteria in cold-stored milk. J Microbiol 42: 133-138.
  33. Yoon KY, Woodams EE, Hang YD. 2004. Probiotication of tomato juice by lactic acid bacteria. J Microbiol 42: 315-318.
  34. Shin KS, Kiyohara H, Matsumoto T, Yamada H. 1997.Rhamnogalacturonan II from the leaves of Panax ginseng C.A. Meyer as a macrophage Fc receptor expression-enhancing polysaccharide. Carbohydr Res 300: 239-249.
  35. Kim HS, Lee EH, Ko SR, Choi KJ, Park JH, Im DS.2004. Effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells. Arch Pharm Res 27:429-435.
  36. Park EK, Choo MK, Han MJ, Kim DH. 2004. Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities.Int Arch Allergy Imm 133: 113-120.
  37. Park YC, Lee CH, Kang HS, Kim KW, Chung HT, KimHD. 1996. Ginsenoside-Rh1 and Rh2 inhibit the induction of nitric oxide synthesis in murine peritoneal macrophages. Biochem Mol Biol Int 40: 751-757.
  38. Lee SJ, Ko WG, Kim JH, Sung JH, Moon CK, Lee BH. 2000. Induction of apoptosis by a novel intestinal metabolite of ginseng saponin via cytochrome c-mediated activation of caspase-3 protease. Biochem Pharmacol 60: 677-685.
  39. Kudo K, Tachikawa E, Kashimoto T, Takahashi E. 1998.Properties of ginseng saponin inhibition of catecholamine secretion in bovine adrenal chromaffin cells. Eur J Pharmacol341: 139-144.

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