Study on Selective Media for Isolation of Entomopathogenic Fungi

  • Shin, Tae-Young (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Choi, Jae-Bang (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Bae, Sung-Min (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Koo, Hyun-Na (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Woo, Soo-Dong (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University)
  • Received : 2009.12.08
  • Accepted : 2010.02.05
  • Published : 2010.03.31

Abstract

To determine the optimal isolation conditions of the entomopathogenic fungi from soil, we compared their growth characteristics with non-entomopathogenic fungi on agar media containing various concentrations of cooper (II) chloride ($CuCl_2$) or dodine. The result showed that dodine medium is more selective, and the optimal concentration of dodine is determined with $50{\mu}g$/ml. We could isolate several putative entomopathogenic fungi from soil using this, and identify them using ITS analysis. As a result, 64% fungi were identified as typical entomopathogenic fungi. This selective medium may be useful to the rapid and simple isolation of entomopathogenic fungi from soil.

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