- Volume 23 Issue 8
DOI QR Code
Gene Expression of Heart and Adipocyte Fatty Acid-binding Protein in Chickens by FQ-RT-PCR
- Tu, Yunjie (Poultry Institute, Chinese Academy of Agricultural Sciences) ;
- Su, Yijun (Poultry Institute, Chinese Academy of Agricultural Sciences) ;
- Wang, Kehua (Poultry Institute, Chinese Academy of Agricultural Sciences) ;
- Zhang, Xueyu (Poultry Institute, Chinese Academy of Agricultural Sciences) ;
- Tong, Haibing (Poultry Institute, Chinese Academy of Agricultural Sciences) ;
- Gao, Yushi (Poultry Institute, Chinese Academy of Agricultural Sciences)
- Received : 2009.11.03
- Accepted : 2010.02.11
- Published : 2010.08.01
This study was to detect the expression of heart fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) gene mRNA in different tissues of Rugao and Luyuan chickens at 56 d and 120 d by real-time fluorescence quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR). The primers were designed according to the sequences of HFABP, A-FABP and GAPDH genes in Gallus gallus, which were used as target genes and internal reference gene, respectively. The levels of H-FABP and A-FABP gene expression were detected by SYBR Green I FQ-RT-PCR. The relative H-FABP and A-FABP gene mRNA expression level was calculated with 2-
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